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Evidence that a light accelerated abrupt change in membrane characteristics of bovine rod outer segment fragments takes place at acidic ph






Biochemical & Biophysical Research Communications 95(3): 1119-1125

Evidence that a light accelerated abrupt change in membrane characteristics of bovine rod outer segment fragments takes place at acidic ph

Changes in the turbidity of suspensions of bovine rod outer segment fragments induced by rhodopsin bleaching were measured in various concentrations of divalent cations at acidic pH (4.7-5.4). The turbidity of the suspensions increased drastically by bleaching at acidic pH, unlike at neutral pH. The extent of turbidity change became maximum at a particular concentration of divalent cations (i.e., 5mM CaCl2, 5 mM MgCl2 or 5 mM mixed divalent cations). The turbidity increment in the presence of 5 mM MgCl2 was greatly enhanced by the addition of a minute amount of CaCl2. The membrane characteristic apparently is abruptly changed by bleaching at acidic pH. Two kinds of binding sites for Ca ions apparently exist: a Ca2+ specific site and a nonspecific site to which Mg2+ can bind.

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Accession: 005411998

PMID: 7417302



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