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Exogenous secretory proteins do not inhibit carbamyl choline stimulated release of pulse labeled secretory proteins from rat pancreatic tissue slices


Biochimica et Biophysica Acta 632(1): 106-111
Exogenous secretory proteins do not inhibit carbamyl choline stimulated release of pulse labeled secretory proteins from rat pancreatic tissue slices
Experiments were conducted to test the concept that the results of secretion studies employing pancreatic tissue slices are significantly biased by the distribution of exocrine secretory proteins between the tissue and the incubation medium. Release kinetics of pulse-labeled secretory proteins from cholinergically-stimulated tissue slices are apparently independent of the concentration of exogenous exocrine secretory proteins. If there are bidirectional fluxes of secretory proteins across the cell membrane of pancreatic exocrine cells, these fluxes apparently do not account for the fractional release of pulse-labeled secretory proteins.


Accession: 005419119

PMID: 7417514



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