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Experimental amoebiasis part 3 a rapid in vitro assay for virulence of entamoeba histolytica



Experimental amoebiasis part 3 a rapid in vitro assay for virulence of entamoeba histolytica



American Journal of Tropical Medicine and Hygiene 27(5): 882-887



A rapid and simple assay for cytopathogenicity of axenically cultivated E. histolytica was developed employing baby hamster kidney (BHK) or mouse 3T3 [Swiss mouse fibroblast] cells conventionally tissue cultured. Of the 12 amebal strains tested 3 produced total destruction of the BHK cell monolayer in 2-3 h; these 3 strains were the most virulent for the newborn hamster liver. Two additional strains were of moderate cytopathogenicity in vitro and of moderate virulence in vivo. Seven strains were of low cytopathogenicity and virulence. Within these 3 major groupings, cytopathogenicity ranking was not entirely reproducible. The general correspondence of cytopathogenicity in vitro and virulence in vivo and the possibility of obtaining data within a few hours suggest such an assay may prove a useful tool in amebiasis research.

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Accession: 005421265

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