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Experimental lung lesion in mice by intra venous administration of soluble immune complexes


Journal of the Osaka City Medical Center 29(2): 341-358
Experimental lung lesion in mice by intra venous administration of soluble immune complexes
Soluble immune complexes consisting of rabbit antibody to bovine serum albumin (BSA) were made up at 5-fold and 20-fold antigen excess. Preformed 5-fold antigen excess BSA anti-BSA immune complexes were given twice i.v. to C57BL/6 mice at an interval of 6 h. An acute interstitial pneumonia, characterized by interstitial mononuclear cell infiltration, edema and congestion, was induced 24 h after the 1st injection. Similar histopathological changes were observed after 6 h when immune complexes were administered twice at a 3 h interval. Even at 30 min after the injection, similar but less prominent pulmonary changes were observed. Seventy-two hours after the 1st injection, the pulmonary inflammatory changes almost disappeared. Immunofluorescence showed that the antigen (BSA) and the antibody (rabbit .gamma.-globulin) located in alveolar capillary walls and infiltrating mononuclear cells. The mouse C3 [complement component 3] was observed in similar fashion although the immunofluorescence was very weak. When immune complexes of 20-fold antigen excess were given, the pulmonary inflammatory changes were very weak as compared with the changes elicited by the 5-fold antigen excess immune complexes. Inactivation of the mouse C system with the treatment of cobra venom factor showed no suppressive effect on the inflammatory changes caused by 5-fold antigen excess immune complexes. In vitro, the 5-fold antigen excess immune complexes had a much stronger C fixing activity than the 20-fold antigen excess immune complexes.


Accession: 005424322



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