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Experimental transmission to calf and goat of the virus associated with the cultured t cell lymphoma cell line from calf type of bovine leukosis i. detection of antibodies to bovine t cell lymphoma cell associated antigen by immunofluorescence assay



Experimental transmission to calf and goat of the virus associated with the cultured t cell lymphoma cell line from calf type of bovine leukosis i. detection of antibodies to bovine t cell lymphoma cell associated antigen by immunofluorescence assay



Bulletin of the Nippon Veterinary and Animal Science University (34): 23-26



Both a calf and a goat of 3 months old were inoculated subcutaneously with bovine T-lymphoid tumor cell line (BTL-C3), which was associated with herpesvirus-like particles (BTLV). Changes of the serum antibody titres to the BTL cell associated antigen (BTLA) were determinated respectively in the calf and goat by the indirect immunofluorescence assay. About goat after the inoculation, the anti-BTLA antibody titres of the sera, which were absorbed with normal bovine thymus cells, were compared with those of the non-absorbed sera. Anti-BTLA antibody titres in the absorbed goat sera were highly positive (1:160) a month after the inoculation. After 2 months, the antibody titre decreased (1:40), but they were still positive (1:10 .apprx. 1:20) over 6 months. The antibodies in non-absorbed goat sera were produced continuously with the higher titres (1:80 .apprx. 1:160) over 6 months. About calf , the anti-BTLA antibody titre in absorbed serum was not different from that in non-absorbed sera. The antibody titres in the calf sera were highly positive (1:80) a month after the inoculation, but dropped to the low level (1:20) after 2 months. However, they rised again to the high levels (1:80) after 4 months and were positive over 5 months. The two positive peak reactions were considered to be a feature of the infection of the virus. These results indicated that the BTLV infected to the calf and goat, and the BTLA on the tumor cells were certain components of the virus.

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