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Experimental transmission to calf and goat of the virus associated with the cultured t cell lymphoma cell line from calf type of bovine leukosis ii. detection of antibodies to bovine t cell lymphoma cell associated virus by elisa



Experimental transmission to calf and goat of the virus associated with the cultured t cell lymphoma cell line from calf type of bovine leukosis ii. detection of antibodies to bovine t cell lymphoma cell associated virus by elisa



Bulletin of the Nippon Veterinary and Animal Science University (34): 27-31



The lymphoma cell line (BTL-C3) derived from calf type of bovine leukosis which produced herpesvirus-like particles was cultured. Infectivities to a calf and a goat of the BTL cell-associated virus (BTLV) were examined in this study. The BTLV was inoculated to the calf and the goat for the experimental transmission and induction of the antibodies to the virus. For the detection of the antibodies to BTLV, the enzyme-linked immunosorbent assay (ELISA) was developed. For ELISA, the BTLV antigen was prepared from the crude virus fraction that was precipitated by ultracentrifuge from the culture supernatant of BTL-C3 cells. The goat antisera were absorbed with normal bovine thymocytes and used for ELISA. As the results in the sera of the calf and the goat inoculated with BTLV, the specific antibodies to BTLV were detected by ELISA. In the calf, the antibodies were increased 1 to 4 months after the inoculation, and they were still positive 5 months after the inoculation, although they were slightly decreased. In the goat, the antibodies were already highly positive a month after the inoculation. Two to 3 months after the inoculation, the antibodies were decreased, but they were increased 4 to 5 months after the inoculation. Then, the antibodies were continuously positive over 7 months after the inoculation. From these results, we concluded that BTLV was infectious to the calf and the goat.

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