+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Experiments on cervical embryo transfer in cattle effects of paper sheath covering transfer instruments on pregnancy rates and uterine bacterial contaminations



Experiments on cervical embryo transfer in cattle effects of paper sheath covering transfer instruments on pregnancy rates and uterine bacterial contaminations



Japanese Journal of Animal Reproduction 28(1): 30-35



Prophylactic effects of paper sheath covering the transfer instruments during and after cervical passages for embryo transfer on pregnancy rate and on the degree of bacterial contamination were examined. In experiment 1, embryos collected from 7 superovulated donor heifers 7-8 days after estrus were grouped into 2 categories and transferred to the appropriate recipients using a metal AI instrument (0.25 ml capacity straw) covered with (group 1) or without (group 2) paper sheath. A total of 46 single cervical transfers ipsilateral to the corpus luteum were performed by the same operator. Pregnancy in the recipient heifers was determined by rectal palpations repeated twice on 33-35 days and 55-59 days after the transfer. Pregnancy rates following the transfers were 63.6% (14/22) in group 1 and 33.3% (8/24) in group 2. There were significant differences between the 2 groups (.chi.2 = 4.224, P < 0.05). In experiment 2, cervical passages of the transfer instrument were carried out by the same operator as in experiment 1 for 20 heifers 7-9 days after estrus. Cervical transfer instruments were inserted into the vagina and the uterus, either covered with (group 1) or without (group 2) the paper sheath. After withdrawals of the instrument from the uterus and the vagina, the content of the straw which loaded in the instrument was released into a sterile test tube. Contaminations of mucus and mucosa were found in all collected media. Bacterial contamination in the media after aerobic and anaerobic incubation for 48-72 h at 37.degree. C were more frequent in group 2 than in group 1. Thus, uterine bacterial contamination may take place with the use of AI instrument for cervical transfer. Reduction of such contamination by the paper sheath might cause the improvement of the pregnancy rate.

(PDF emailed within 1 workday: $29.90)

Accession: 005428404

Download citation: RISBibTeXText


Related references

Middle to lower uterine segment embryo transfer improves implantation and pregnancy rates compared with fundal embryo transfer. Fertility and Sterility 81(5): 1273-1277, 2004

Ultrasonographic-guided embryo transfer does not enhance pregnancy rates compared with embryo transfer based on previous uterine length measurement. Fertility and Sterility 86(4): 867-872, 2006

Stimulation of endometrium embryo transfer (SEET): injection of embryo culture supernatant into the uterine cavity before blastocyst transfer can improve implantation and pregnancy rates. Fertility and Sterility 88(5): 1339-1343, 2007

Comparison of pregnancy and implantation rates in zygote intrafallopian transfer and uterine embryo transfer for nontubal infertility. Eastern Mediterranean Health Journal 16(1): 29-33, 2010

Comparison of concurrent pregnancy rates for in-vitro fertilization--embryo transfer, pronuclear stage embryo transfer and gamete intra-fallopian transfer. Human Reproduction 5(8): 947-954, 1990

Effects of PGR and ESRĪ± genotypes on the pregnancy rates after embryo transfer in Luxi cattle. Molecular Biology Reports 40(1): 579-584, 2013

Effects of bovine somatotropin and timed embryo transfer on pregnancy rates in non-lactating cattle. Veterinary Record 156(6): 175-176, 2005

Frozen embryo transfer instead of fresh embryo transfer can increase pregnancy and implantation rates and reduce biochemical pregnancy in advanced maternal aged women. Reproductive Biomedicine Online 35: E3-E4, 2017

Single blastocyst embryo transfer maintains comparable pregnancy rates to double cleavage-stage embryo transfer but results in healthier pregnancy outcomes. Australian and New Zealand Journal of Obstetrics and Gynaecology 51(5): 406-410, 2012

Injection of day 2 embryo culture supernatant into the uterine cavity did not improve the pregnancy rate of day 3 embryo transfer in patients who underwent in vitro fertilization-embryo transfer: a randomized clinical trial. Fertility and Sterility 93(7): 2216-2221, 2010

The difference in pregnancy rates between elective single embryo transfer (SET) compared to double embryo transfer is dependent on the implantation rates of embryos being transferred. Using mathematical modeling to determine when SET becomes a viable option. Human Reproduction 21(8): 2195; Author Reply 2195-6, 2006

Blood on the embryo transfer catheter is associated with decreased rates of embryo implantation and clinical pregnancy with the use of in vitro fertilization-embryo transfer. Fertility & Sterility 70(5): 878-882, 1998

Effects in cattle of genetic variation within the IGF1R gene on the superovulation performance and pregnancy rates after embryo transfer. Animal Reproduction Science 143(1-4): 24-29, 2014

Uterine peristalsis before embryo transfer affects the chance of clinical pregnancy in fresh and frozen-thawed embryo transfer cycles. Human Reproduction 29(6): 1238-1243, 2015

Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenology 5, 56(2): 287-297, 2001