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Experiments on cervical embryo transfer in cattle effects of paper sheath covering transfer instruments on pregnancy rates and uterine bacterial contaminations

Experiments on cervical embryo transfer in cattle effects of paper sheath covering transfer instruments on pregnancy rates and uterine bacterial contaminations

Japanese Journal of Animal Reproduction 28(1): 30-35

Prophylactic effects of paper sheath covering the transfer instruments during and after cervical passages for embryo transfer on pregnancy rate and on the degree of bacterial contamination were examined. In experiment 1, embryos collected from 7 superovulated donor heifers 7-8 days after estrus were grouped into 2 categories and transferred to the appropriate recipients using a metal AI instrument (0.25 ml capacity straw) covered with (group 1) or without (group 2) paper sheath. A total of 46 single cervical transfers ipsilateral to the corpus luteum were performed by the same operator. Pregnancy in the recipient heifers was determined by rectal palpations repeated twice on 33-35 days and 55-59 days after the transfer. Pregnancy rates following the transfers were 63.6% (14/22) in group 1 and 33.3% (8/24) in group 2. There were significant differences between the 2 groups (.chi.2 = 4.224, P < 0.05). In experiment 2, cervical passages of the transfer instrument were carried out by the same operator as in experiment 1 for 20 heifers 7-9 days after estrus. Cervical transfer instruments were inserted into the vagina and the uterus, either covered with (group 1) or without (group 2) the paper sheath. After withdrawals of the instrument from the uterus and the vagina, the content of the straw which loaded in the instrument was released into a sterile test tube. Contaminations of mucus and mucosa were found in all collected media. Bacterial contamination in the media after aerobic and anaerobic incubation for 48-72 h at 37.degree. C were more frequent in group 2 than in group 1. Thus, uterine bacterial contamination may take place with the use of AI instrument for cervical transfer. Reduction of such contamination by the paper sheath might cause the improvement of the pregnancy rate.

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Accession: 005428404

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