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Expression of nicotinic acetylcholine receptor in oocytes of xenopus laevis


Expression of nicotinic acetylcholine receptor in oocytes of xenopus laevis



Biologia (Bratislava) 43(4): 289-296



ISSN/ISBN: 0006-3088

Polyadenylated mRNAs from denervated rat muscle have been isolated, analysed and separated by gradient centrifugation. Translational activity of 18-29S mRNAs has been tested in the rabbit reticulocyte lysate and in Xenopus laevis oocytes. Expression of nicotinic acetylcholine receptors was monitored after microinjection of oocytes by 125I .alpha.-bungarotoxin binding and by in vitro labelling of receptors synthesized in the oocyte membranes.

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Accession: 005431455

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