Expression of nuclear and chloroplastic genes coding for fraction 1 protein in somatic hybrids of nicotiana tabacum cultivar bright yellow and nicotiana rustica var rustica

Iwai, S.; Nagao, T.; Nakata, K.; Kawashima, N.; Matsuyama, S.

Planta (Heidelberg) 147(5): 414-417


ISSN/ISBN: 0032-0935
Accession: 005431472

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In the sexual interspecific cross, N. rustica L. .times. N. tabacum L., N. rustica can serve as the female but not as the male parent. By fusion of protoplasts, the barrier to fertilization was overcome and somatic hybrids containing N. tabacum cytoplasm were produced as shown by isoelectric focusing of the Fraction-1 protein (F-1-protein). All somatic hybrids displayed polypeptides of the large subunit of F-1 protein (which is coded by the chloroplast genome) characteristic of only one or the other parental species. Two hybrids had large subunits of the N. tabacum type and 2 hybrids had those of the N. rustica type. Three hybrids contained 3 small-subunit polypeptides (coded by the nuclear genome), one being characteristic of N. rustica, 1 characteristic of N. tabacum, and one with an isoelectric point common to both species. A 4th hybrid contained only 2 small-subunit polypeptides of the N. tabacum type but in a F-1 protein macromolecule whose large subunits were of the N. rustica type. One somatic hybrid was self-fertile and its F2 progeny contained large- and small-subunit polypeptides indistinguishable in their isoelectric points from those in the parent F1 hybrid. All somatic hybrids showed an aneuploid chromosome number and morphological characteristics intermediate between those of N. rustica and N. tabacum.