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Expression of the h 2 antigenic complex on murine hematopoietic stem cells


, : Expression of the h 2 antigenic complex on murine hematopoietic stem cells. Scandinavian Journal of Immunology 15(6): 547-552

Hybridoma-derived monoclonal antibody to H-2Kk antigens and xenoantibodies to .beta.2-microglobulin (.beta.2m) were used to study the expression of the H-2 antigenic molecular complex on murine hematopoietic stem cells, and the effect of long-term culture in vitro on the expression of these antigens. Monoclonal anti-H-2Kk antibody produced potent complement-dependent inhibition of pluripotent (CFU-S), myeloid (CFU-C), and erythroid (CFU-E) stem cells from the bone marrow of C3H and AKR (H-2k) mice, and was without effect on stem cells from Balb/C (H-2d) mice. Anti-.beta.2m xenoantibodies inhibited stem cells from all 3 strains. Stem cells could not be rescued from the inhibitory effects of the antibodies by the addition of thymocytes to marrow cells after antibody treatment. Both the anti-H-2Kk monoclonal antibody and the anti-.beta.2m xenoantibodies produced potent inhibition of AKR (H-2k) CFU-C that was maintained in culture for up to 6 wk. Murine CFU-S, CFU-C and CFU-E express H-2 antigens, and the expression of these antigens by CFU-C is not altered during long-term culture.

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Lushnikov, V. P., 1996: Increasing the production and improving the quality of sheep meat in the Volga region. Production of sheep meat in the region accounts for about 10% of total production in Russia. Studies on improvement of the production and quality of meat by breeding programmes are reviewed. The bibliography is not printed in the journal.

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