EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of the Hantaan virus M genome segment by using a vaccinia virus recombinant


Journal of Virology 62(3): 696-702
Expression of the Hantaan virus M genome segment by using a vaccinia virus recombinant
A cDNA containing the complete open reading frame of the Hantaan virus (HTN) M genome segment has been cloned into vaccinia virus. This recombinant virus expresses two glycoproteins which are similar to the HTN structural glycoproteins, G1 and G2, in molecular weight, cleavage pattern, and cellular distribution. Both HTN and recombinant vaccinia virus glycoproteins are exclusively associated with the Golgi apparatus of the cell. Despite this intracellular restriction, mice inoculated with the recombinant vaccinia virus raised neutralizing antibodies against HTN. The specificity of virus neutralization appears to reside in the HTN glycoproteins, since a vaccinia virus recombinant expressing the HTN nucleocapsid protein was unable to elicit a neutralizing antibody response.


Accession: 005432008

PMID: 3123711



Related references

Expression of truncated regions of the S genome segment of Hantaan virus in E. coli and the antigen of the recombinant proteins. Chinese Journal of Zoonoses 15(6): 67-70, 1999

Expression strategy of the M genome segment of Hantaan virus. Virus Research. 31(1): 109-121, 1994

Expression strategy of the M genome segment of Hantaan virus. Virus Research 31(1): 109-121, 1994

A vaccinia virus-vectored Hantaan virus vaccine protects hamsters from challenge with Hantaan and Seoul viruses but not Puumala virus. Journal of Virology 69(10): 6417-6423, 1995

Expression of the S genome segment of Hantaan virus 76-118 in E. coli: Evaluation of antigenicity of the capsid protein. Journal of Bacteriology & Virology 31(2): 187-192, June, 2001

Nucleocapsid- and virus-like particles assemble in cells infected with recombinant baculoviruses or vaccinia viruses expressing the M and the S segments of Hantaan virus. Virus Research. 38(2-3): 111-124, 1995

Nucleocapsid- and virus-like particles assemble in cells infected with recombinant baculoviruses or vaccinia viruses expressing the M and the S segments of Hantaan virus. Virus Research 38(2-3): 111-124, 1995

Effect of in vitro mutations in a vaccinia virus early promoter region monitored by herpes simplex virus thymidine kinase expression in recombinant vaccinia virus. Journal of General Virology 68: 2299-2309, 1987

Baculovirus expression of the small genome segment of Hantaan virus and potential use of the expressed nucleocapsid protein as a diagnostic antigen. Journal of General Virology 69: 777-786, 1988

Nucleotide sequence of the L genome segment of Hantaan virus. Nucleic Acids Research 18(22): 6728-6728, 1990