EurekaMag.com logo
+ Translate

Expression of the hepatitis b virus genome in the oocyte of 2 amphibian species


, : Expression of the hepatitis b virus genome in the oocyte of 2 amphibian species. Biology of the Cell (Paris) 50(3): 223-228

Xenopus laevis and Pleurodeles waltlii oocyte nuclei are injected with a recombinant plasmid pCP 10, carrying 2 hepatitis B virus (HBV) genomes in a tandem arrangement. Transcription occurs in oocytes of both species and production of viral RNA is demonstrated; better quantitative results are obtained with P. waltlii oocytes. Two viral proteins are detected, hepatitis B surface antigen and hepatitis B core antigen. These proteins can be shown only in oocyte homogenates, and not in the incubation media. Another viral marker was not detected.

(PDF 0-2 workdays service)

Accession: 005432013

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Larive-Zerbani, A.; Gallien, C.L.; Pourcel, C., 1984: Expression of hepatitis B virus (HBV) genome in the oocytes of two species of amphibians. Xenopus laevis and Pleurodeles waltlii oocyte nuclei are injected with a recombinant plasmid pCP 10, carrying two hepatitis B virus (HBV) genomes in a tandem arrangement. Transcription occurs in oocytes of both species and production of viral RNAs...

Wu, P.C.; Fang, J.W.; Lai, C.L.; Lau, S.S.; Lo, C.K.; Lai, A.; Lau, J.Y., 1996: Hepatic expression of hepatitis B virus genome in chronic hepatitis B virus infection. The expression of hepatitis B virus (HBV) DNA in the liver was studied by nonisotopic in situ hybridization and correlated with liver histology, different phases in the natural evolution of chronic hepatitis B, and hepatic expression of HBV antige...

Anon, 1980: Integration of the virus genome into the cellular genome during serum hepatitis b and some characteristics of expression of viral genes. Voprosy Virusologii (4): 498

Han, K.H.; Oh, S.H.; Chon, C.Y.; Moon, Y.M.; Park, I.S.; Park, Y.R.; Park, C.I., 1995: Hepatitis B virus genome and genomic expression in the liver tissue of the patients with hepatitis B virus related chronic liver diseases. Gastroenterology 108(4 SUPPL ): A1080

Bowyer, S.M.; Dusheiko, G.M.; Schoub, B.D.; Kew, M.C., 1987: Expression of the hepatitis B virus genome in chronic hepatitis B carriers and patients with hepatocellular carcinoma. We examined the methylation status of CCGG sites in hepatitis B virus (HBV) DNA to determine whether methylation could be responsible for the selective expression of the HBV surface gene in chronic hepatitis B infection and hepatocellular carcinom...

Lau J.Y.N.; Portmann B.C.; Naoumouv N.V.; Alexander G.J.M.; Williams R., 1991: A novel pattern of hepatitis b virus hbv genome expression associated with fibrosing cholestatic hepatitis fch in liver grafts. Hepatology 14(4 PART 2): 284A

Anonymous, 1985: Presence and expression of hepatitis b virus genome in mononuclear cells of patients with type b hepatitis. Journal of Hepatology 1

Tanaka, T.; Shimotohno, K., 1995: Expression of the proteins encoded by the hepatitis C viral genome by vaccinia virus expression system. Nihon Rinsho. Japanese Journal of Clinical Medicine 53 Suppl(Pt 1): 85-90

Li, Y.; Wasser, S.; Lim, S.G.; Tan, T.M.C., 2004: Genome-wide expression profiling of RNA interference of hepatitis B virus gene expression and replication. Small interfering RNA (siRNA) has been used repeatedly to down-regulate viral gene expression and inhibit viral replication in mammalian cells. In this study, we showed that siRNAs specific for two conserved regions within the hepatitis B S antige...

Nishihara, T.; Mizuno, K.; Shikata, T., 1993: Expression of hepatitis C virus genome. Complementary DNAs from HCV genome were expressed and analyzed. C8-2 protein, a part of NS5, and core protein were synthesized in E. coli. Core protein (JCC) was appeared to be very useful for diagnostic probe of HCV infection. Predicted envelope...