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Expression of the hepatitis b virus genome in the oocyte of 2 amphibian species


, : Expression of the hepatitis b virus genome in the oocyte of 2 amphibian species. Biology of the Cell (Paris) 50(3): 223-228

Xenopus laevis and Pleurodeles waltlii oocyte nuclei are injected with a recombinant plasmid pCP 10, carrying 2 hepatitis B virus (HBV) genomes in a tandem arrangement. Transcription occurs in oocytes of both species and production of viral RNA is demonstrated; better quantitative results are obtained with P. waltlii oocytes. Two viral proteins are detected, hepatitis B surface antigen and hepatitis B core antigen. These proteins can be shown only in oocyte homogenates, and not in the incubation media. Another viral marker was not detected.

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