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Expression of the hepatitis b virus surface antigen gene in cell culture by using a sv 40 vector

, : Expression of the hepatitis b virus surface antigen gene in cell culture by using a sv 40 vector. Proceedings of the National Academy of Sciences of the United States of America 78(4): 2606-2610

A SV40 recombinant carrying a fragment of DNA from hepatitis B virus was constructed. Cultured African green monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22 nm particles with the same physical properties, antigenic composition and constituent polypeptides as those found in the sera of patients with type B hepatitis.

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