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Expression of the human amylase genes: recent origin of a salivary amylase promoter from an actin pseudogene


, : Expression of the human amylase genes: recent origin of a salivary amylase promoter from an actin pseudogene. Nucleic Acids Research 16(17): 8261-8276

The human genes encoding salivary amylase (AMY1) and pancreatic amylase (AMY2) are nearly identical in structure and sequence. We have used ribonuclease protection studies to identify the functional gene copies in this multigene family. Riboprobes derived from each gene were hybridized to RNA from human pancreas, parotid and liver. The sizes of the protected fragments demonstrated that both pancreatic genes are expressed in pancreas. One of the pancreatic genes, AMY2B, is also transcribed at a low level in liver, but not from the promoter used in pancreas. AMY1 transcripts were detected in parotid, but not in pancreas or liver. Unexpected fragments protected by liver RNA led to the discovery that the 5' regions of the five human amylase genes contain a processed .gamma.-actin pseudogene. The promoter and start site for transcription of AMY1 are recently derived from the 3' untranslated region of .gamma.-actin. In addition, insertion of an endogenous retrovirus has interrupted the .gamma.-actin pseudogene in four of the five amylase genes.

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Accession: 005432028

PMID: 2458567

DOI: 10.1093/nar/16.17.8261

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Bhandari N.; Murty S. V. S.; Shukla P. N.; Mahajan R. R.; Shukla A. D.; Suthar K. M.; Parthasarathy G.; Paliwal B. S., 2005: Bhawad LL6 chondrite; chemistry, petrology, noble gases, nuclear tracks, and cosmogenic radionuclides. Chemical and mineral analysis of the Bhawad chondrite, which fell in Rajasthan in 2002, suggest that this stone belongs to LL6 group of chondrites. Based on helium, neon, and argon isotopes, it has a cosmic ray exposure age of 16.3 Ma. The track d...

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Lajtha A.; Banay Schwartz M.; Kenessey A., 1991: Changes in brain protease levels under various conditions. Journal of Cellular Biochemistry Supplement (15 PART G): 133

Burini, P.; Ciani, A., 1994: Analysis of production costs for forage crops. A survey of forage production costs in Umbria, Italy, is reported; the study aimed to estimate the difference that exists between production cost and market price of forage crops and to investigate alternative crops and techniques of production an...

Mellen Frederic Francis, 1936: The bentonite deposits of Mississippi. Rocks and Minerals 11(10): 220-222

Bezuglova T.V.; Gusman B.S., 1980: Experimental study of ultrastructural changes in the lungs in mycoplasma pneumoniae infection. Ultrastructural changes in the lungs were studied in aerosol infected (M. pneumoniae) Syrian hamsters. The pathological process began with an intensive vascular reaction followed by a very speedy interstitial and serous-desquamative pneumonia with...

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