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Expression of the macrolide lincosamide streptogramin b resistance methylase gene erm e from streptomyces erythraeus in escherichia coli results in n 6 monomethylation and n 6 n 6 dimethylation of ribosomal rna

, : Expression of the macrolide lincosamide streptogramin b resistance methylase gene erm e from streptomyces erythraeus in escherichia coli results in n 6 monomethylation and n 6 n 6 dimethylation of ribosomal rna. Gene (Amsterdam) 55(2-3): 319-326

The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6-dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.

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