EurekaMag.com logo
+ Translate

Expression of the macrolide lincosamide streptogramin b resistance methylase gene erm e from streptomyces erythraeus in escherichia coli results in n 6 monomethylation and n 6 n 6 dimethylation of ribosomal rna


, : Expression of the macrolide lincosamide streptogramin b resistance methylase gene erm e from streptomyces erythraeus in escherichia coli results in n 6 monomethylation and n 6 n 6 dimethylation of ribosomal rna. Gene (Amsterdam) 55(2-3): 319-326

The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6-dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.

(PDF 0-2 workdays service)

Accession: 005432095

PMID: 3117622

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Katz, L.; Brown, D.; Boris, K.; Tuan, J., 1987: Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N6-monomethylation and N6,N6-dimethylation of ribosomal RNA. The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS)...

Uchiyama H.; Weisblum B., 1985: Macrolide lincosamide streptogramin b resistance in streptomyces erythreus sequence of the 23s ribosomal rna methylase that mediates resistance and its similarity to other macrolide lincosamide streptogramin resistance methylases. Leive, L (Ed ) Microbiology- ; Meetings Of The American Society For Microbiology X+486p American Society For Microbiology: Washington, D C , Usa Illus 375-378

Jin, H.J., 1999: ermSF, a ribosomal RNA adenine N6-methyltransferase gene from Streptomyces fradiae, confers MLS (macrolide-lincosamide-streptogramin B) resistance to E. coli when it is expressed. The Erm family of methyltransferases confers the MLS antibiotic resistance to pathogenic microorganism through the mono- or dimethylation of a single adenine residue in 23S rRNA, which is known as the target site for modification. One of the erm g...

Hardy, K.; Haefeli, C., 1982: Expression in Escherichia coli of a staphylococcal gene for resistance to macrolide, lincosamide, and streptogramin type B antibiotics. Plasmid pBD9, which comprises two plasmids from Staphylococcus aureus, pE194 and pUB110, was joined to plasmid pBR322 by in vitro recombination to form plasmid pKH80. The ermC gene of plasmid pE194 confers inducible resistance to macrolide, lincos...

Uchiyama H.; Weisblum B., 1985: N methyltransferase of streptomyces erythraeus that confers resistance to the macrolide lincosamide streptogramin b antibiotics amino acid sequence and its homology to cognate r factor enzymes from pathogenic bacilli and cocci. The nucleotide sequence of a structural gene ermE for ribosomal RNA (rRNA) N6-amino adenine N-methyl transferase (NMT) of Streptomyces erythraeus, cloned by Thompson et al. [Gene 20 (1982) 51-62], has been determined. The NMT amino acid (aa) seque...

Uchiyama, H.; Weisblum, B., 1985: N-Methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci. The nucleotide sequence of a structural gene ermE for ribosomal RNA (rRNA) N6-amino adenine N-methyl transferase (NMT) of Streptomyces erythraeus, cloned by Thompson et al. [Gene 20 (1982) 51-62], has been determined. The NMT amino acid (aa) seque...

Halula, M.C.; Manning, S.; Macrina, F.L., 1991: Nucleotide sequence of ermFU, a macrolide-lincosamide-streptogramin (MLS) resistance gene encoding an RNA methylase from the conjugal element of Bacteroides fragilis V503. Nucleic Acids Research 19(12): 3453-3453

Fujisawa, Y.; Weisblum, B., 1981: A family of r-determinants in Streptomyces spp. that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics. Inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics in Streptomyces spp. comprises a family of diverse phenotypes in which characteristic subsets of the macrolide-lincosamide-streptogramin antibiotics induce resist...

Burridge, R.; Warren, C.; Phillips, I., 1986: Macrolide, lincosamide and streptogramin resistance in Campylobacter jejuni/coli. Antibiotic sensitivity/resistance patterns were studied in seven animal strains of C. coli naturally resistant to erythromycin (five porcine strains, one ovine and one chicken strain) and in 22 human strains of C. coli and 2 human strains of C. je...

Werner, G.; Hildebrandt, B.; Klare, I.; Witte, W., 2000: Linkage of determinants for streptogramin A, macrolide-lincosamide-streptogramin B, and chloramphenicol resistance on a conjugative plasmid in Enterococcus faecium and dissemination of this cluster among streptogramin-resistant enterococci. A new streptogramin A resistance gene, satG (= vatE), has been recently identified in Enterococcus faecium UW1965 (Werner and Witte 1999. Antimicrob. Agents Chemother. 43: 1813-1814). Further sequence analysis of this plasmid revealed that vatE is...