EurekaMag.com logo
+ Translate

Expression of the messenger rna for tau proteins during brain development and in cultured neurons and astroglial cells


, : Expression of the messenger rna for tau proteins during brain development and in cultured neurons and astroglial cells. Rousset, B A F (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 171 La Structure Et Les Fonctions Du Cytosquelette: Aspects Biologiques Et Physiopathologiques; (Inserm (National Institute Of Health And Medical Research) Colloquium), Vol 171 Structure And Functions Of The Cytoskeleton: Biological And Physiopathological Aspects; Symposium, Lyon, France, April 13-16, Xvi+585p Les Editions Inserm: Paris, France; John Libbey And Co Ltd : Lon

Two .tau. cDNA probes of 1.6 and 0.3 kilobases (kb) have been used to study the expresion of the .tau. mRNAs during mouse brain development and in highly homogeneous primary cultures of neurons and astrocytes. (1) Whatever the stage, a 6-kb mRNA was detected with the two probes. In the astrocytes a 6-kb mRNA hybridized clearly only with the 1.6-kb probe. (2) During brain development the abundance of .tau. mRNA increases from a late fetal stage (-4 days) until birth, remains high until 6 days postnatal, and then markedly decreases to reach very low values in adulthood. Such a marked decrease in the abundance of .tau. mRNA parallels that of .alpha.-tubulin mRNA. These data suggest that: (1) depending on the stage of development and on the cell type (neurons or astrocytes) .tau. mRNAs of the same size encode several .tau. proteins differing in molecular weight: several .tau. proteins are expressed either during early stages of development (juvenile .tau. proteins of 48 kilodaltons) or in adulthood (mature .tau. proteins of 50-70 kilodaltons) or are specific of the astrocyte (83 kilodaltons). (2) The expression of the two major components of axonal microtubules, tubulin and .tau. proteins, seems to be developmentally coordinated.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432117

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Couchie, D.; Charrière-Bertrand, C.; Nunez, J., 1988: Expression of the mRNA for tau proteins during brain development and in cultured neurons and astroglial cells. Two tau cDNA probes of 1.6 and 0.3 kilobases (kb) have been used to study the expression of the tau mRNAs during mouse brain development and in highly homogeneous primary cultures of neurons and astrocytes. (1) Whatever the stage, a 6-kb mRNA was...

Köller, H.; Schaal, H.; Rosenbaum, C.; Czardybon, M.; Von Giesen, H.Jürgen.; Müller, H.Werner.; Arendt, G., 2002: Functional CXCR4 receptor development parallels sensitivity to HIV-1 gp120 in cultured rat astroglial cells but not in cultured rat cortical neurons. The alpha chemokine receptor CXCR4 is used as the major coreceptor for the cell entry of T-cell-tropic human immunodeficiency virus-1 (HIV-1) isolates. Activation of this coreceptor by its natural ligand SDF1alpha is associated with an intracellul...

Schousboe A., 1987: Cultured neurons and astroglial cells as model systems to study development and differentiation. Acta Neurologica Scandinavica 76(3): 232

Kranich, O.; Hamprecht, B.; Dringen, R., 1996: Different preferences in the utilization of amino acids for glutathione synthesis in cultured neurons and astroglial cells derived from rat brain. The intracellular contents of glutathione in neuron-rich and astroglia-rich primary cultures derived from the brains of embryonal and newborn rats were found to be 23.1 +- 3.0 and 31.2 +- 6.5 nmol/mg of protein, respectively. Deprivation of amino...

Broer, S.; Rahman, B.; Pellegri, G.; Pellerin, L.; Martin, J.-Luc; Verleysdonk, S.; Hamprecht, B.; Magistretti, P., J., 1997: Comparison of lactate transport in astroglial cells and monocarboxylate transporter 1 (MCT 1) expressing Xenopus laevis oocytes: Expression of two different monocarboxylate transporters in astroglial cells and neurons. The transport of lactate is an essential part of the concept of metabolic coupling between neurons and glia. Lactate transport in primary cultures of astroglial cells was shown to be mediated by a single saturable transport system with a Km value...

Bröer, S.; Rahman, B.; Pellegri, G.; Pellerin, L.; Martin, J.L.; Verleysdonk, S.; Hamprecht, B.; Magistretti, P.J., 1997: Comparison of lactate transport in astroglial cells and monocarboxylate transporter 1 (MCT 1) expressing Xenopus laevis oocytes. Expression of two different monocarboxylate transporters in astroglial cells and neurons. The transport of lactate is an essential part of the concept of metabolic coupling between neurons and glia. Lactate transport in primary cultures of astroglial cells was shown to be mediated by a single saturable transport system with a Km value...

Reuss, B.; Hertel, M.; Werner, S.; Unsicker, K., 2000: Fibroblast growth factors-5 and -9 distinctly regulate expression and function of the gap junction protein connexin43 in cultured astroglial cells from different brain regions. Astroglial cells contribute to neuronal maintenance and function in the normal and diseased brain. Gap junctions formed predominantly by connexin43 (cx43) provide important pathways to coordinate astroglial responses. We have previously shown that...

Hirota, N.; Matsumoto, K.; Iida, M.; Sakagami, H.; Takeda, M., 1998: Expression of somatostatin messenger RNA and receptor in cultured brain tumor cells. In order to understand the role of somatostatin in tumor cell growth, the expression of somatostatin in 7 cultured brain tumor cells was investigated. Radioimmunoassay demonstrated the production of somatostatin into the culture supernatant of 6 t...

Nothias, F.; Boyne, L.; Murray, M.; Tessler, A.; Fischer, I., 1995: The expression and distribution of tau proteins and messenger RNA in rat dorsal root ganglion neurons during development and regeneration. Microtubule-associated proteins contribute to the balance between stability and plasticity of the neuronal cytoskeleton by modulating assembly and disassembly of microtubules. The tau microtubule-associated proteins exist in several isoforms which...

Nothias, F.; Boyne, L.; Murray, M.; Tessler, A.; Fischer, I., 1995: The expression and distribution of tau proteins and messenger RNA in rat dorsal root ganglion neurons during development and regeneration. Microtubule-associated proteins contribute to the balance between stability and plasticity of the neuronal cytoskeleton by modulating assembly and disassembly of microtubules. The tau microtubule-associated proteins exist in several isoforms which...