EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of the microsomal antigen on the surface of continuously cultured rat thyroid cells is modulated by thyrotropin






Journal of Clinical Endocrinology and Metabolism 61(1): 12-16

Expression of the microsomal antigen on the surface of continuously cultured rat thyroid cells is modulated by thyrotropin

The thyroid microsomal antigen, commonly implicated in thyroid autoimmune diseases, is not confined to cytoplasm, but is also present on the surface of human thyroid cells in primary culture. In this study, a strain of differentiated rat thyroid cells (FRTL5) grown in continuous culture was used to investigate the relationship between functional status of the thyroid cell and expression of microsomal antigen on its surface. Sera from eight patients with Hashimoto's thyroiditis, one with idiopathic hypothyroidism, and three with hyperthyroid Graves' disease were selected for the presence of microsomal antibody, but undetectable thyroglobulin antibody. One additional serum sample from a patient with Graves' disease, which was negative for both thyroid antibodies was used. Sera from eight normal subjects were used as controls. Indirect immunofluorescence was applied to FRTL5 cells that were viable or fixed with acetone in order to expose intracellular antigens. When FRTL5 cells were cultured in Coon's medium containing TSH (300 microU/ml), positive indirect immunofluorescence surface staining was observed with sera containing microsomal antibody, but not with control sera or with the Graves' serum negative for microsomal antibody. A rough correlation was found between the intensity of the surface fluorescence and microsomal antibody titer. After acetone treatment, microsomal antibody-positive sera produced typical cytoplasmic staining. When FRTL5 cells were cultured in the absence of TSH, disappearance of the surface fluorescence and reduction of the cytoplasmic microsomal antigen occurred. Readdition of TSH progressively restored both the surface and cytoplasmic microsomal antigen. The present data indicate that the thyroid microsomal antigen is represented on the surface of FRTL5 cells, and its expression is modulated by TSH.


Accession: 005432119

PMID: 3889033

DOI: 10.1210/jcem-61-1-12



Related references

Vitti P.; Chiovato L.; Ceccarelli P.; Lombardi A.; Novaes M.Jr; Fenzi G.F.; Pinchera A., 1986: Thyroid stimulating antibody mimics thyrotropin in its ability to desensitize the cyclic amp response to acute stimulation in continuously cultured rat thyroid cells frt l 5. TSH preincubation induces refractoriness to further stimulation of cAMP accumulation by the hormone in cultured thyroid cells. The question of whether the thyroid-stimulating antibodies (TSAb) present in sera of patients with Graves' disease...

Vitti, P.; Chiovato, L.; Ceccarelli, P.; Lombardi, A.; Novaes, M.; Fenzi, G.F.; Pinchera, A., 1986: Thyroid-stimulating antibody mimics thyrotropin in its ability to desensitize the adenosine 3',5'-monophosphate response to acute stimulation in continuously cultured rat thyroid cells (FRT-L5). TSH preincubation induces refractoriness to further stimulation of cAMP accumulation by the hormone in cultured thyroid cells. The question of whether the thyroid-stimulating antibodies (TSAb) present in sera of patients with Graves' disease...

Chiovato L.; Vitti P.; Cucchi P.; Mammoli C.; Carajon P.; Pinchera A., 1989: The expression of the microsomal peroxidase autoantigen in human thyroid cells is thyrotropin dependent. In the present report the mechanisms responsible for the expression of the thyroid microsomal autoantigen (M-Ag) were studied in primary cultures of human thyroid cells prepared from Graves' or non-toxic goitres. The indirect immunofluorescen...

Nagayama, Y.; Yamashita, S.; Hirayu, H.; Izumi, M.; Uga, T.; Ishikawa, N.; Ito, K.; Nagataki, S., 1989: Regulation of thyroid peroxidase and thyroglobulin gene expression by thyrotropin in cultured human thyroid cells. cDNAs for thyroid peroxidase (TPO) and thyroglobulin (Tg) have been cloned and sequenced. Using such cDNAs, we investigated the regulation of TPO and Tg gene expression by various agents in cultured human thyroid cells. Unstimulated human thyroid...

Saito, T.; Endo, T.; Kawaguchi, A.; Ikeda, M.; Nakazato, M.; Kogai, T.; Onaya, T., 1997: Increased expression of the Na+/I- symporter in cultured human thyroid cells exposed to thyrotropin and in Graves' thyroid tissue. The Na+/I- symporter (NIS) is important in hormone synthesis in the thyroid gland. NIS activity, as reflected by I- uptake, was increased by TSH (1 mU/mL) or forskolin (10 mu-mol/L) in primary cultured human thyroid cells. Northern blot analysis r...

Chiovato, L.; Vitti, P.; Lombardi, A.; Ceccarelli, P.; Cucchi, P.; Marcocci, C.; Carayon, P.; Pinchera, A., 1988: Studies on the mechanism responsible for thyrotropin-induced expression of microsomal/peroxidase antigen in FRTL-5 cells. The expression of the microsomal (M) antigen on the surface and in the cytoplasm of a strain of rat thyroid cells (FRTL-5) is under the regulation of TSH. In the present report the mechanism by which TSH induces such expression was investigated wi...

Chiovato, L.; Lapi, P.; Mariotti, S.; Del Prete, G.; D.C.rli, M.; Pinchera, A., 1994: Simultaneous expression of thyroid peroxidase and human leukocyte antigen-DR by human thyroid cells: modulation by thyrotropin, thyroid-stimulating antibody, and interferon-gamma. Major histocompatibility class II molecules human leukocyte antigen-DR (HLA-DR) are abnormally expressed by human thyroid cells (HTC) in autoimmune thyroid glands. The simultaneous expression of HLA-DR and organ-specific autoantigens such as thyro...

Shouval D.; Wands J.R., 1985: Modulation of hepatitis b surface antigen expression on the cell surface of human hepatoma cells in culture continuously exposed to monoclonal hepatitis b surface antibodies. Journal of Hepatology (SUPPL 1): S130

Islam, N.; Tuppal, R.; Hawe, B.S.; Briones-Urbina, R.; Farid, N.R., 1986: The thyroid "microsomal" antigen is an epitope on the thyrotropin receptor. Antimicrosomal antibodies are present in the sera of most patients with autoimmune thyroiditis, and Graves' disease. It has, in general, been difficult to separate antimicrosomal activity from that directed against the thyrotropin (TSH) recep...

Chabaud, O.; Chebath, J.; Mauchamp, J., 1981: Thyroglobulin gene expression in cultured thyroid cells: regulation by thyrotropin. The influence of thyrotropin (TSH) on thyroglobulin (Tgb) biosynthesis has been studied porcine thyroid cells maintained in primo culture. After 4 days of culture and 1-hr labelling with 35S Met, Tgb specific radioactivity was 10 times higher in T...