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Expression of the mouse di hydro folate reductase complementary dna in sv 40 vectors

, : Expression of the mouse di hydro folate reductase complementary dna in sv 40 vectors. Molecular & Cellular Biology 1(9): 854-864

A mouse complementary DNA segment coding for the enzyme dihydrofolate reductase was cloned in 2 general classes of vectors containing SV40 DNA: those that can be propagated as virions in permissive cells and those that can be introduced into and maintained stably in various mammalian cells. Both types of vectors express the mouse dihydrofolate reductase by using signals supplied by SV40 DNA nucleic acid sequences. Plasmid vectors carrying the complementary DNA segment can complement Chinese hamster ovary cells lacking dihydrofolate reductase.

Accession: 005432127

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Related references

Nunberg J.H.; Kaufman R.J.; Chang A.C.Y.; Erlich H.A.; Cohen S.N.; Schimke R.T., 1979: Structure of di hydro folate reductase genes in methotrexate resistant mouse lines expression of mouse di hydro folate reductase in escherichia coli. Journal of Supramolecular Structure 8(SUPPL 3): 69

Simonsen, C.C.; Levinson, A.D., 1983: Isolation and expression of an altered mouse di hydro folate reductase ec complementary dna. A c[complementary]DNA library was constructed from a murine cell line expressing high levels of a dihydrofolate reductase (tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC that displays an abnormally low a...

Lee F.; Mulligan R.; Berg P.; Ringold G., 1981: Gluco corticoids regulate expression of di hydro folate reductase complementary dna in mouse mammary tumor virus chimeric plasmids. Nature (London) 294(5838): 228-232

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Cheng Y C., 1984: Activity of the new anti folate n 10 propargyl 5 8 dideaza folate and its poly glutamates against human di hydro folate reductase human thymidylate synthetase and kb cells containing different levels of di hydro folate reductase. Federation Proceedings: Abstract 3890

Kaufman R.J.; Sharp P.A., 1982: Construction of a modular di hydro folate reductase complementary dna gene analysis of signals utilized for efficient expression. Dihydrofolate reductase (DHFR) modular genes were constructed with segments containing the adenovirus major late promoter, a 3' splice site from a variable region Ig gene, a DHFR c[complementary]DNA, and portions of the SV40 genome. DNA-media...

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