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Expression of the mouse serum albumin gene introduced into differentiated and dedifferentiated rat hepatoma cells

, : Expression of the mouse serum albumin gene introduced into differentiated and dedifferentiated rat hepatoma cells. Proceedings of the National Academy of Sciences of the United States of America 82(3): 765-769

A 23-kilobase-pair semgent of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5' and 4.3 kilobase pairs of 3' flanking sequences was introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase c[complementary]DNA is under the control of SV 40 sequences. This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. Only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. A transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell.

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Accession: 005432132

PMID: 3856230

DOI: 10.2307/25352

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Khella, A.K., 1992: Epidemiologic study of premenstrual symptoms. The frequency of selected premenstrual symptoms among 342 Egyptian women were analyzed in relation to demographic and reproductive characteristics. The frequency ranged from 36% for breast pain or tenderness to 66% for premenstrual stomach and bac...

FURUYA, Y., 1961: Genetics of patternless finger dermatoglyph. Jinrui Idengaku Zasshi. Japanese Journal of Human Genetics 6: 102-117

Gavrilescu, S.; Luca, C., 1976: Pseudo-retrograde conduction in complete A-V heart block due to ectopic His bundle activity. A case with complete A-V heart block in whom retrograde P- waves following some of the ventricular complexes were due to an ectopic junctional activity with the characteristics of parasystole is reported. His bundle electrogram showed an infrahisi...

Beyaz, Sülent.Gökhan., 2016: Comparison of transforaminal and interlaminar epidural steroid injections for the treatment of chronic lumbar pain. A cross-sectional study. we compared the 12 month outcomes of fluoroscopically guided transforaminal epidural steroid injections with interlaminar epidural steroid injections for the treatment of chronic lumbar spinal pain. Chronic lower back pain...

Ahlstrom, O.; Skrede, A., 1997: Liver fatty acid composition and peroxisomal fatty acid oxidase activity in blue foxes (Alopex lagopus) and mink (Mustela vison) fed diets containing different levels of fish oil. Differences in the ability to metabolize n-3 fatty acids originating from fish oil was examined in blue fox and mink by monitoring total lipid and phospholipid composition, and peroxisomal beta -oxidation activity in the liver. In experiment 1, 3...

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Yang Fengcai,.; Mao Xueming,.; Zheng Jianqiang,.; Qiu Yuqing,, 1994: Preliminary study on the niche of two wheat aphids and their natural enemies. Entomological Journal of East China ; 3(2): 69-72

Schroeter S.; Lasky C.J.; Oakley R.A.; Erickson C.A.; Tosney K.W., 1990: Evidence for the delineation of neural crest migration pathways by inhibitory boundaries. Society for Neuroscience Abstracts 16(1): 313

Okamoto, M.; Inoue, K.; Iwamura, H.; Terashima, K.; Soya, H.; Asashima, M.; Kuwabara, T., 2011: Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis. The mammalian brain contains neural stem cells (NSCs) that enable continued neurogenesis throughout adulthood. However, NSC function and/or numbers decline with increasing age. Adult hippocampal neurogenesis is unique in that astrocytes secreting...