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Expression of the mouse serum albumin gene introduced into differentiated and dedifferentiated rat hepatoma cells

, : Expression of the mouse serum albumin gene introduced into differentiated and dedifferentiated rat hepatoma cells. Proceedings of the National Academy of Sciences of the United States of America 82(3): 765-769

A 23-kilobase-pair semgent of DNA containing the entire mouse serum albumin gene as well as 2.2 kilobase pairs of 5' and 4.3 kilobase pairs of 3' flanking sequences was introduced into pSV2dhfr, a plasmid in which expression of the mouse dihydrofolate reductase c[complementary]DNA is under the control of SV 40 sequences. This vector, pSV2dhfr-alb, was used to transfect differentiated and variant dedifferentiated rat hepatoma cells. Nine independent clones of transfected differentiated cells secrete considerable amounts of mouse albumin, while the expression of the normal rat albumin is the same as in nontransfected cells. Only small amounts of mouse and rat albumin are produced by transfected dedifferentiated cells. The amounts of albumin mRNA present in the cells are consistent with the amounts of albumin produced. A transfected gene can be regulated in a fashion consistent with the overall differentiation profile of the cell.

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Accession: 005432132

PMID: 3856230

DOI: 10.2307/25352

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Harlow H.F.; Harlow M.K., 1970: Developmental aspects of emotional behavior. Black, Perry (Ed ) Physiological Correlates Of Emotion Xvi+309p Illus Academic Press: New York, N Y , U S A ; London, England 37-58

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Walsh, K.; Schimmel, P., 1988: DNA-binding site for two skeletal actin promoter factors is important for expression in muscle cells. Two nuclear factors bind to the same site in the chicken skeletal actin promoter. Mutations in the footprint sequence which eliminate detectable binding decrease expression in transfected skeletal muscle cells by a factor of 25 to 50 and do not el...

Kosnett, M.J.; Wedeen, R.P.; Rothenberg, S.J.; Hipkins, K.L.; Materna, B.L.; Schwartz, B.S.; Hu, H.; Woolf, A., 2007: Recommendations for medical management of adult lead exposure. Research conducted in recent years has increased public health concern about the toxicity of lead at low dose and has supported a reappraisal of the levels of lead exposure that may be safely tolerated in the workplace. In this article, which appe...

Husseini R.H.; Sweet C.; Collie M.H.; Smith H., 1981: Relation of interferon and nonspecific inhibitors to virus levels in nasal washes of ferrets infected with influenza viruses of differing virulence. Two clones (7a, virulent; 64d, attenuated) of a recombinant influenza virus (A/PR/8/34-A/England/939/69 (H3N2)) were inactivated at the same rate by viral inhibitors present in nasal washes taken from both clone 7a- and clone 64d-infected ferrets....

Solntsev, M.K.; Gribova, Z.P.; Tashish, V.; Karavaev, V.A.; Bairamov, K.B., 1994: Thermoluminescence of Barbarea vulgaris leaves in a wide range of irradiation temperatures and its changes by diuron treatment. Laboratory trials were conducted in Russia to assess the effect of diuron (at 10-5 M) on leaf stalks of Barbarea vulgaris as a function of temperature of irradiation in the area from -80 to 0 degrees C using the thermoluminescence (TL) methods and...

Verma, S.; Verma, G.K.; Singh, G.; Kanga, A.; Shanker, V.; Singh, D.; Gupta, P.; Mokta, K.; Sharma, V., 2012: Sporotrichosis in sub-himalayan India. Sporotrichosis is endemic in the Sub-Himalayan belt, which ranges from the northern to the north-eastern Indian subcontinent. Similar to many parts of the developing world, sporotrichosis is commonly recognized clinically in this region however co...

POPOV, G.M., 1950: History of military-field therapy in Russia. Sovetskaia Meditsina 7: 37-39

Morris, Je, 1991: Managing Iowa fisheries: intensive aquaculture systems. PM Iowa State University Cooperative Extension Service: 91 (1352f)

Yackerson, N.S.; Piura, B.; Friger, M., 2007: The influence of weather state on the incidence of preeclampsia and placental abruption in semi-arid areas. Being close to the big deserts of the Sahara and Saudi Arabia, the Negev desert in the south of Israel is meteorologically defined as a semi-arid area. To investigate the influence of meteorological factors typical for the semi-arid areas on the i...