EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of the n gene in isolated tobacco nicotiana tabacum protoplasts


Fiziologiya Rastenii (Moscow) 33(1): 159-164
Expression of the n gene in isolated tobacco nicotiana tabacum protoplasts
The effect of infection with tobacco mosaic virus was examined on the viability of protoplasts isolated from the leaf mesophyll of Nicotiana tabacum L., cv. Samsun (a sensitive host) and cv. Xanthi nc. (a hypersensitive host). Either the plants were infected before protoplast isolation, or the protoplasts were infected with the virus after isolation. The protoplasts isolated from diseased Xanthi nc. plants during emergence of necrosis were of low viability, but when the protoplasts were infected after isolation, they remained viable. Therefore, the expression of the gene of necrotic response (the N gene) in the first case is suggested to be induced by inoculated leaves just before protoplast isolation. No decrease in viability was observed in the protoplasts isolated from the leaves of healthy or diseased Samsun plants, as well as from the leaves of healthy Xanthi nc. plants or from the leaves with developed necrosis. The N gene was not induced if the infected Xanthi nc. plants were kept at 32.degree. , and the protoplasts from those leaves were not destroyed. The role of tissue integrity, the existence of cell walls in particular, in the N gene expression is discussed.


Accession: 005432142



Related references

Expression of the n gene in plasmolyzed leaf tissues and isolated protoplasts of nicotiana tabacum cultivar xanthi nc infected by tobacco mosaic virus. Kiraly, Z (Ed ) Current Topics in Plant Pathology Proceedings Of A Symposium Budapest, Hungary, June 24-27, 1975 443p Illus Akademiai Kiado: Budapest, Hungary Isbn 963-05-1171-1 395-398, 1977

Expression of the N-gene in plasmolysed leaf tissues and isolated protoplasts of Nicotiana tabacum cv. Xanthi nc. infected by TMV. Current topics in plant pathology [2]: 395-398, 1977

Viability of protoplasts isolated from tobacco leaves (Nicotiana tabacum, var. Samsun) infected by tobacco mosaic virus. Zesz Probl Postepow Nauk Roln (174): 11-16, 1976

Viability of protoplasts isolated from tobacco leaves (Nicotiana tabacum variety Samsun) infected with tobacco mosaic virus. 1976

Inhibition of tobacco mosaic virus reproduction in isolated tobacco protoplasts by means of pancreatic ribonculease Nicotiana tabacum, antiviral acitvity. Phytopathologische Zeitschrift = Journal of phytopathology 106(1): 35-44, 1983

Phenyl alanine ammonia lyase levels in protoplasts isolated from hyper sensitive tobacco nicotiana tabacum cultivar samsun nm pre infected with tobacco mosaic virus. Planta (Heidelberg) 146(4): 451-458, 1979

The penetration of tritiated dna of bacillus subtilis into isolated protoplasts of cells of tobacco nicotiana tabacum. Doklady Biological Sciences 219(1-6): (1975), 1974

Penetration of tritium labeled dna of bacillus subtilis into isolated protoplasts of cells of tobacco nicotiana tabacum. Doklady Akademii Nauk SSSR Seriya Biologiya 219(6): 1478-1481, 1974

Regeneration of virus-free plants from protoplasts isolated from dark-green areas of tobacco mosaic virus-infected tobacco leaves Nicotiana tabacum. Plant tissue culture 1982: proceedings 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake Japan July 11-16-1982 edited by Akio Fujiwara: 804, 1982

Co expression and inheritance of foreign genes in transformants obtained by direct dna transformation of tobacco nicotiana tabacum protoplasts. Molecular & General Genetics 205(1): 1-8, 1986