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Expression of the normal human cis platelet derived growth factor 2 coding sequence induces cellular transformation

, : Expression of the normal human cis platelet derived growth factor 2 coding sequence induces cellular transformation. Cell 39(1): 89-98

The human sis proto-oncogene contains the coding sequence for 1 of 2 polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH/3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kilobase sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. The number coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.

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