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Expression of the phosphoenolpyruvate carboxykinase gene and its insulin regulation during differentiation of preadipose cell lines

, : Expression of the phosphoenolpyruvate carboxykinase gene and its insulin regulation during differentiation of preadipose cell lines. Biochemical & Biophysical Research Communications 138(1): 468-475

The adipose conversion of Ob1771 and 3T3-F442A preadipose cells is accompanied by the expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene. The PEPCK mRNA is absent from growing, undifferentiated Ob1771 and 3T3-F442A cells as well as from non-differentiating 3T3-C2 cells. It is present in differential Ob1771 and 3T3-F442A cells as well as in liver, kidney and white adipose tissue from mouse. Transcriptional run-off measurements in nuclei isolated from undifferentiated and differentiated Ob1771 and 3T3-F442A cells reveal that the PEPCK gene transcription is activated during differentiation. Studies of the time course of changes indicate that the emergence of PEPCK mRNA takes place in parallel to mRNA encoding for a 28 kDA protein (28 K mRNA) but later than that encoding for glycerol-3-phosphate dehydrogenase (GPDH mRNA). Insulin leads to an increase in the content of PEPCK and GPDH mRNAs with half-maximally effective concentrations of 0.5 and 5 nM for GPDH mRNA and PEPCK mRNA, respectively. Thus, in contrast to rat hepatoma cells, insulin exerts in adipose cells a positive regulation on the expression of the PEPCK gene.

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Accession: 005432162

PMID: 3527164

DOI: 10.1016/0006-291x(86)90304-9

PDF Full Text: Expression of the phosphoenolpyruvate carboxykinase gene and its insulin regulation during differentiation of preadipose cell lines

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Bache, D. H., 1980: Transport and capture processes within plant canopies. An absorption coefficient, representing the rate of attenuation of an aerosol cloud as it passes through a plant canopy was analysed in terms of a range of particle diameters, canopy windspeed, and characteristic dimensions typical of foliar eleme...

Zhang Sun, G.; Yang, C.R.n; Viallet, J.; Feng, G.S.eng; Bergeron, J.J.M.; Posner, B.I., 1996: A 60-kilodalton protein in rat hepatoma cells overexpressing insulin receptor was tyrosine phosphorylated and associated with Syp, phosphatidylinositol 3-kinase, and Grb2 in an insulin-dependent manner. Tyrosine phosphorylation of cellular proteins is an early and key step after activation of the insulin receptor kinase (IRK). The study of the properties of these proteins should contribute to our understanding of insulin action. In rat hepatoma c...

Kim, H.; Kataru, R.P.; Koh, G.Young., 2014: Inflammation-associated lymphangiogenesis: a double-edged sword?. Lymphangiogenesis and lymphatic vessel remodeling are complex biological processes frequently observed during inflammation. Accumulating evidence indicates that inflammation-associated lymphangiogenesis (IAL) is not merely an endpoint event, but a...

Rupert, P.B.; Daughdrill, G.W.; Bowerman, B.; Matthews, B.W., 1998: A new DNA-binding motif in the Skn-1 binding domain-DNA complex. The DNA-binding domain of Skn-1, a developmental transcription factor that specifies mesoderm in C. elegans, is shown by X-ray crystallography to have a novel fold in which a compact, monomeric, four-helix unit organizes two DNA-contact elements....

Il'ichev V.G., 1983: Stability of model with delay as illustrated by phyto plankton phosphorus. Biophysics (English Translation of Biofizika) 27(4): 736-740

Villavicencio Mavrich, H., 1996: Toward modern uro-oncology. Actas Urologicas Espanolas 20(4): 309-313

Dono, M-Josée.; Gaudron, M., 2006: Opening of the interregional secure hospital unit in Nancy..., the nurses' point of view. Soins; la Revue de Reference Infirmiere: 38-39

Morrisey, J.; Hardesty, B., 1972: Met-tRNA hydrolase from reticulocytes specific for Met-tRNA f Met on 40S ribosomal subunits. Archives of Biochemistry and Biophysics 152(1): 385-397

Li, L.C.; Zhang, Y.S.; Hu, R.Z.; Zhou, X.C., 1992: Inhibitory effect of fluoride on renal stone formation in rats. The effect of fluorine (F) on stone formation induced by ethylene glycol (EG) was studied in rats. For different groups, the drinking water was supplemented with EG, sodium fluoride (NaF), EG+NaF, or nothing as control. An isotope-tracing method w...

Broomfield, L.M.; Bochmann, M.; Wright, J.A., 2012: Tris[3,6-di-tert-butyl-1-(isoquinolin-1-yl)naphthalen-2-olato-κ2N,O]aluminium(III) toluene sesquisolvate. The central Al(III) atom of the title compound, [Al(C(27)H(28)NO)(3)]·1.5C(7)H(8), has octahedral geometry in which the three N atoms are arranged in a meridional fashion. One of the toluene solvent molecules is located on a general position, whi...