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Expression of the platelet derived growth factor related transforming protein of simian sarcoma virus in escherichia coli


, : Expression of the platelet derived growth factor related transforming protein of simian sarcoma virus in escherichia coli. Cell 36(1): 43-50

The transforming gene of simian sarcoma virus (SSV), an acute transforming retrovirus, and human platelet-derived growth factor (PDGF), a potent mitogen for connective tissue cells, appear to have arisen from the same or very closely related celluar genes. In an effort to obtain sufficient quantities of the SSV transforming gene product for the detailed analysis of its structural and biologic properties, the v-sis gene was placed under the control of strong phage transcriptional and translational signals that provide for regulated expression of cloned genes in E. coli. When induced, the bacterial cells synthesized levels of the SSV transforming gene product that constituted at least 10% of their total protein. Differences in the structure and processing of the v-sis gene product in prokaryotic and eukaryotic cells provided important insights concerning posttranslational modifications of this PDGF-related transforming protein in eukaryotic cells.

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Betsholtz C.; Heldin C H.; Nister M.; E.B.; Wasteson A.; Westermark B., 1983: Synthesis of a platelet derived growth factor like growth factor in human glioma and sarcoma cells suggests the expression of the cellular homologue to the transforming protein of simian sarcoma virus. Several human normal and neoplastic cell lines were screened for production of PDGF [platelet-derived growth factor] receptor competing activity. Conditioned medium from 2 sarcomas [U-20S, RD] and 1 glioma [U-343 MGA clone 2] blocked 125I-PDGF bin...

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