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Expression of the platelet derived growth factor related transforming protein of simian sarcoma virus in escherichia coli

, : Expression of the platelet derived growth factor related transforming protein of simian sarcoma virus in escherichia coli. Cell 36(1): 43-50

The transforming gene of simian sarcoma virus (SSV), an acute transforming retrovirus, and human platelet-derived growth factor (PDGF), a potent mitogen for connective tissue cells, appear to have arisen from the same or very closely related celluar genes. In an effort to obtain sufficient quantities of the SSV transforming gene product for the detailed analysis of its structural and biologic properties, the v-sis gene was placed under the control of strong phage transcriptional and translational signals that provide for regulated expression of cloned genes in E. coli. When induced, the bacterial cells synthesized levels of the SSV transforming gene product that constituted at least 10% of their total protein. Differences in the structure and processing of the v-sis gene product in prokaryotic and eukaryotic cells provided important insights concerning posttranslational modifications of this PDGF-related transforming protein in eukaryotic cells.

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Chattopadhyay S.K.; Lowy D.R.; Teich N.M.; Levine A.S.; Rowe W.P., 1975: Qualitative and quantitative studies of akr type murine leukemia virus sequences in mouse dna. Ford, Nancy (Ed ) Cold Spring Harbor Symposia on Quantitative Biology, Vol 39 Tumor Viruses Xxvi+688p (Vol 1 ); Xvi+545p (Vol 2) Illus Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y, U S A Isbn 0-87969-038-0 1085-1101

Zajonc, I., 1957: Neue Erkenntnisse uber die Regenwurmer der Beskyden (Oligochaeta-Lumbricidae). Prirodovedecky Sbornik Ostravskeho Kraje, 18: 161-169

Schwab, Stefan; Hacke, Werner, 2003: Surgical decompression of patients with large middle cerebral artery infarcts is effective. Stroke 34(9): 2304-2305

Watts, Ralph N.; Ponka, Prem; Richardson, Des R., 2003: Effects of nitrogen monoxide and carbon monoxide on molecular and cellular iron metabolism: Mirror-image effector molecules that target iron. Many effector functions of nitrogen monoxide (NO) and carbon monoxide (CO) are mediated through their high-affinity for iron (Fe). In this review, the roles of NO and CO are examined in terms of their effects on the molecular and cellular mechanis...

Anonymous, 1992: The regulation of biotechnologies with emphasis on the release of genetically modified organisms to the environment. This publication summarizes the discussions and transcribes 15 papers presented at the Second Meeting of the Interamerican Group of Studies on New Biotechnologies, held in Brasilia, Brazil, during 29 May and 1 June 1990, as part of the IICA's...

Jamieson, Pd; Wilson, Dr, 1985: Modelling the influence of irrigation and drought on crop production and water use. Les Besoins en eau des cultures = Crop water requirements: conf int Versailles France 11-14 sept 1984 tenue au siege de l'Unesco avec le concours de l'OAA et de l'OMM = held at Unesco under auspices of FAO and WMO: 443

Beaussart M.; Beaussart Boulenge L., 1970: Experimental study of cerebral concussion in 123 amateur boxers by clinical examination and electro encephalogram before and immediately after fights. Electroencephalography & Clinical Neurophysiology 29(5): 529-530

Anonymous, 2013: Early Magnetic Resonance Detection of Natalizumab-Related Progressive Multifocal Leukoencephalopathy in a Patient with Multiple Sclerosis. Diagnosis of progressive multifocal leukoencephalopathy is usually based on the clinical presentation, on the demonstration of the brain lesions at the magnetic resonance imaging examination, and on the detection of the JC virus DNA in the cerebro...

Kraemer, U.; Illig, T.; Grune, T.; Krutmann, J.; Esser, C., 2007: Strong associations of psoriasis with antigen processing LMP and transport genes TAP differ by gender and phenotype. Psoriasis, a skin disease with autoimmune features, can be triggered and exacerbated by genetic and environmental factors. Chemicals can break tolerance to self-antigens by interfering with antigen processing and presentation; therefore, proteins...

Ohno, Mizuki; Tenzen, Toyoaki; Watanabe, Yoshihisa; Yamagata, Tetsushi; Kanaya, Shigehiko; Ikemura, Toshimichi, 2000: Non-B DNA structures spatially and sequence-specifically associated with individual centromeres in the human interphase nucleus. Olmo, Ettore [Author], Redi, Carlo Alberto [Author] Chromosomes Today 57-69