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Expression of the proenkephalin gene in human neuroblastoma cell lines


, : Expression of the proenkephalin gene in human neuroblastoma cell lines. Molecular Brain Research 3(2): 147-154

Several human tumour cell lines were screened for secretion of proenkephalin-derived peptides with an antiserum directed to its N-terminus. Met-enkephalin-Arg6,Phe7 and for proopiomelanocortin-derived peptides with an antiserum to .beta.-endorphin. The neuroblastoma SK-N-MC cell line secreted Met-enkephalin-ARg6,Phe7-immunoreactive peptides in relatively high amounts into the culture medium, although processing was not complete and there was no evidence for free Met-enkephalin-Arg6,Phe7. Gene expression was confirmed by the presence of proenkephalin mRNA and proenkephalin-derived polypeptides in extracts of the SK-N-MC cells and also in the neuroblastoma SH-SY5Y cell line. In the latter cells, however, the expression was approximately 3 times lower, there was less processing of proenkephalin and no evidence for secretion.

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Accession: 005432181

PMID: 3382940

DOI: 10.1016/0169-328X(88)90060-5

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