EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of the Proteus mirabilis lipoprotein gene in Escherichia coli. Existence of tandem promoters


, : Expression of the Proteus mirabilis lipoprotein gene in Escherichia coli. Existence of tandem promoters. Journal of Biological Chemistry 261(10): 4600-4606

The lpp gene from Proteus mirabilis was cloned onto pBR322 and expressed in Escherichia coli. The P. mirabilis lpp gene is unique in that it has two tandom promoters transcribing two mRNAs that differ in length by approximately 70 nucleotides at their 5'-ends. The two mRNAs thus encode the identical lipoprotein. The P. mirabilis prolipoprotein has a 19-protein acid signal peptide and a 59-amino acid lipoprotein sequence. In spite of the substantial differences in the amino acid sequence from the E. coli prolipoprotein, the P. mirabilis prolipoprotein is normally modified and processed in E. coli, and the resultant lipoprotein is assembled in the E. coli outer membrane as is the E. coli lipoprotein.


Accession: 005432186

PMID: 3007466

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Stevenson, L.G.; Szostek, B.A.; Clemmer, K.M.; Rather, P.N., 2013: Expression of the DisA amino acid decarboxylase from Proteus mirabilis inhibits motility and class 2 flagellar gene expression in Escherichia coli. In <italic>Proteus mirabilis</italic>, a putative phenylalanine decarboxylase (DisA) acts in a regulatory pathway to inhibit class 2 flagellar gene expression and motility. In this study, we demonstrate that DisA expression in <ital...

Gruner, G.; Tadros, M.H.; Plapp, R., 1985: Isolation of labeled lipoprotein from Escherichia coli and Proteus mirabilis after incubation with [14C]penicillin. [14C]penicillin binding experiments and membrane analysis were carried out with cell envelope preparations from Escherichia coli and Proteus mirabilis. After incubation with [14C]penicillin G labeled free lipoprotein could be identified. The analy...

Gruner G.; Tadros M.H.; Plapp R., 1985: Isolation of labeled lipoprotein from escherichia coli and proteus mirabilis after incubation with carbon 14 penicillin. [14C]Penicillin binding experiments and membrane analysis were carried out with cell envelope preparations from E. coli and P. mirabilis. After incubation with [14C]penicillin G labeled free lipoprotein could be identified. The analysis of the iso...

Smith J.T., 1969: R factor gene expression in gram negative bacteria escherichia coli serratia marcescens alkalescens sp aerobacter aerogenes proteus mirabilis enz penicillinase. Journal of General Microbiology 55(1/2/3): 109-120

Rebel, G.; Bellard, F.; Vincedon, G., 1966: Existence de fractions lipidiques phosphorees dans les lipides lies du Proteus mirabilis P 18 Existence of phosphorus lipid fractions in linked lipids of Proteus mirabilis p 18. Soc Chim Biol Bull 48(1): 179-181

Morgan E.A.; Kaplan S., 1974: The expression of escherichia coli ribosomal dna in proteus mirabilis. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 185

Niebel H.; Stackebrandt E., 1987: Cloning and expression of ribosomal dna cistrons from proteus mirabilis in escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 87: 151

Coker, C.; Mobley, H.L.T., 1998: H-NS influences expression of proteus mirabilis urease when expressed in Escherichia coli. Abstracts of the General Meeting of the American Society for Microbiology 98: 71-72

Roberts, M.; Baumberg, S., 1984: Anomalous expression of the escherichia coli lac operon in proteus mirabilis 1. effects of l 8 and l 8 uv 5. The lac operon shows anomalous expression in P. mirabilis; the maximal induced level is 10% or less of that in E. coli, while repression reduces this by a factor of only 2-5. One sought to determine whether this effect relates in any way to CRP-me...

Morgan, E.A.; Kaplan, S., 1977: Expression and stability of Escherichia coli F-prime factors in Proteus mirabilis. The expression and stability of Escherichia coli F-primes in Proteus mirabilis is examined. It is possible to consecutively introduce, and stably maintain, the DNA of several E. coli F-primes in P. mirabilis in the absence of selective pressure fo...