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Expression of the silent hemo globin gene in sheep studies of the globin messenger rna


, : Expression of the silent hemo globin gene in sheep studies of the globin messenger rna. Journal of Biological Chemistry 247(20): 6665-6670



Accession: 005432224

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Related references

Benz, E.J.Jr ; Barker, J.E.; Pierce, J.E.; Turner, P.A.; Nienhuis, A.W., 1978: Hemo globin switching in sheep commitment of erythroid stem cells to expression of the beta c globin gene and accumulation of beta c globin messenger rna. The switch from HbA .**GRAPHIC**. to HbC .**GRAPHIC**. synthesis was induced by injection of erythropoietin into a lamb homozygous for HbA. Serial samples of bone marrow were analyzed to detect the initial commitment of erythroid stem cells (CFU-E...

Benz, E.J.Jr ; Geist, C.E.; Steggles, A.W.; Barker, J.E.; Nienhuis, A.W., 1977: Hemo globin switching in sheep and goats preparation and characterization of complementary dna specific for the alpha globin beta globin and gamma globin messenger rna of sheep. Specific complementary DNAs (cDNA) for the messenger RNA coding for sheep .alpha.-, .beta.A-, .beta.B-, .beta.C- and .gamma.-globins were prepared by thermal denaturation of heterologous hybrids (e.g., .alpha.beta.b-cDNA.cntdot.alpha.gamma.-mRNA)...

Benz E.J.Jr; Kretschmer P.J.; Geist C.E.; Kantor J.A.; Turner P.H.; Nienhuis A.W., 1979: Hemo globin switching in sheep synthesis cloning and characterization of dna sequences coding for the beta b globin messenger rna beta c globin messenger rna and gamma globin messenger rna. Synthetic double-stranded DNA (sDNA) were prepared from sheep globin mRNA templates isolated from reticulocytes producing either HbB (.alpha.2.beta.B2), HbC (.alpha.2.beta.C2) or HbF (.alpha.2.gamma.2). These sDNA were inserted into the Eco RI sit...

Benz, E.J.Jr ; Steggles, A.W.; Geist, C.E.; Nienhuis, A.W., 1978: Hemo globin switching in sheep quantitation of beta a messenger rna and beta c messenger rna sequences in nuclear and cytoplasmic rna during the hemo globin a to hemo globin c switch. To quantitate changes in nuclear and cytoplasmic .beta.A- and .beta.C-mRNA sequences during switching from HbA (.alpha.2.beta.2A) to HbC (.alpha.2.beta.2C), a molecular hybridization assay was developed which allows specific measurement of the mRN...

Schneider R.G., 1974: Differentiation of electrophoretically similar hemo globins such as hemo globin s hemo globin d hemo globin g and hemo globin p or hemo globin a 2 hemo globin c hemo globin e and hemo globin o by electrophoresis of the globin chains. Clinical Chemistry 20(9): 1111-1115

Baldy, M.; Gaskill, P.; Kabat, D., 1972: Expression of the silent hemoglobin gene in sheep. Studies of the globin messenger ribonucleic acids. A study was made of the ribonucleic acids in reticulocytes of adult sheep, which carry different globin genes and synthesize different haemoglobins. Haemoglobin A( alpha 2 beta 2A) is synthesized by A/A homozygotes in early anaemia, but these anim...

Killion P.J.; Cameron B.F., 1972: Water sorption and vapor phase deuterium exchange studies on met hemo globin cc met hemo globin sc met hemo globin ss met hemo globin as and met hemo globin aa. Biophysical Journal 12(5): 528-539

Nishikura, K.; Sugita, Y.; Nagai, M.; Yoneyama, Y., 1975: Ethyl iso cyanide equilibria of hemo globin m iwate hemo globin m boston hemo globin m hyde park hemo globin m saskatoon and hemo globin m milwaukee i in half ferric and fully reduced states. Journal of Biological Chemistry 250(17): 6679-6685

Charlesworth D., 1975: A new hemo globin variant hemo globin f sardinia gamma 75 e 19 iso leucine replaced by threonine found in a family with hemo globin g philadelphia beta chain deficiency and a lepore like hemo globin indistinguishable from hemo globin a 2. Acta Haematologica (basel): 347-355

Gardiner M.B.; Carver J.; Abraham B.L.; Wilson J.B.; Huisman T.H.J., 1982: Further studies on the quantitation of the hemo globin a hemo globin s hemo globin c and hemo globin f in new born babies with different hemo globinopathies using high pressure liquid chromatography. High pressure liquid chromatography (HPLC) was used for the detection and quantitation of the .beta. chain variants Hb S and Hb C in blood samples of newborn babies with different hemoglobinopathies. The complete separation of the Hb C, S, A and F...