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Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants

Proceedings of the National Academy of Sciences of the United States of America 83(8): 2709-2713

Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants

Transformed cell lines derived from murine C127 cells were constructed that express the influenza virus RNA-dependent RNA polymerase proteins (PA, PB1, and PB2). Cell lines that express only one or all three of the proteins were tested for their ability to complement temperature-sensitive viral mutants incubated at the nonpermissive temperature. Two cell lines were isolated that express all three polymerase genes and complement the growth of PB2 temperature-sensitive mutants at the nonpermissive temperature. One of these lines also complemented PA temperature-sensitive mutants. The viral titers obtained in these two cell lines were 12-fold to 1000-fold higher than the viral titers obtained upon growth of the corresponding temperature-sensitive mutant in C127 cells at the nonpermissive temperature.

Accession: 005432249

PMID: 3010315

DOI: 10.2307/27355

Download PDF Full Text: Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants

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