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Expression of the truncated escherichia coli o 6 methylguanine methyltransferase gene in repair deficient human cells and restoration of cellular resistance to alkylating agents

, : Expression of the truncated escherichia coli o 6 methylguanine methyltransferase gene in repair deficient human cells and restoration of cellular resistance to alkylating agents. Mutation Research 184(2): 121-128

We have constructed a truncated Escherichia coli O6-methylguanine methyltransferase (MT) gene (ada gene) to express the MT activity for O6-methylguanine and O4-methylthymine but not for methylphosphotriester in human cells and transferred in into Mer- HeLa MR cells. The transfectant cells expressed the truncated E. coli MT were resistant to alkylating agents as same as the transfectant cells with the intact ada gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. These results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.

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Ozawa, S.; Sasaya, T.; Tabei, Y., 1988: Extractives of todomatsu Abies sachalinensis Masters VI. Dihydrobenzofurans and tetrahydrofurans from the wood of Abies sachalinensis. An account of the structure of 5 further lignans.

Gu Lei; Zhang Xu; Huang ZhenChun; Fan DongPu, 2006: Application of workflow technology in digital forestry grid. Some applications in digital forestry are data-flow driven. However, no efficient supported environment for data-flow driven applications exists in forestry. A workflow-based technology for digital forestry grid that supports data-flow driven appl...

Lebedev L. M., 1961: Morfologicheskie osobennosti kvartsa i kassiterita Shakh-Shagailinskogo mestorozhdeniya. Quartz and cassiterite are paragenetically associated with several stages of the mineralization of the Shakh-Shagaily tin deposit, Kazakhstan, USSR. The ore occurs in a brecciated greisenized zone in leucocratic granites. Quartz-cassiterite aggreg...

Churakov, Gennady; Smit, Arian, F. A.; Brosius, Juergen; Schmitz, Juergen, 2005: A novel abundant family of retroposed elements (DAS-SINEs) in the nine-banded armadillo (Dasypus novemcinctus). About half of the mammalian genome is composed of retroposons. Long interspersed elements (LINEs) and short interspersed elements (SINEs) are the most abundant repetitive elements and account for about 21% and 13% of the human genome, respectively...

Rao G.H.R.; White J.G., 1973: Measurement of platelet serotonin. Federation Proceedings 32(3 PART 1): 843

Meunier, Francois J., 1984: Spatial organization and mineralization of the basal plate of elasmoid scales in osteichthyans. American Zoologist 24(4 ): 3-64

Hernandez G.; Lifschitz M.; Stein J., 1978: Persistent natriuretic effect of prostaglandin d 2 in the dog. Kidney International 14(6): 762

Granger Harry Clifford, 1956: Dripping Spring quartzite, Arizona. Trace Elements Investigations Report: 204-210. 1956.

Fetlinski, A.; Stepaniak, L., 1991: The trait of resistance to bile salts in Lactobacillus acidophilus, Lactobacillus bulgaricus and Lactobacillus casei strains as indispensable information in the data base for collection of industrial strains. The resistance of selected Lactobacillus casei and Lactobacillus acidophilus strains (from the BIOLACTA collection) to bile salts was determined. Culture lyophylizates or bacteria containing acidophilic tablets were used. Bacteria were cultured on...

Strong W.H., 1986: Restructuring to create greater value. TAPPI (Technical Association of the Pulp & Paper Industry) Journal 69(1): 46-48