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Expression of the vesicular stomatitis virus nucleocapsid protein controlled by the lambda p r and p l promoters in escherichia coli


, : Expression of the vesicular stomatitis virus nucleocapsid protein controlled by the lambda p r and p l promoters in escherichia coli. Journal of Biotechnology 2(5): 289-302

Expression vectors were constructed in which a complementary DNA specifying the vesicular stomatitis virus nucleocapsid (VSV N) protein was inserted near the translational initiation region downstream from the thermoinducible PR or PL promoter of bacteriophage .lambda. Expression of the VSV N-protein was determined by a radioimmunoassay with monoclonal antibody prepared against the VSV N-protein. The expression of the VSV N-protein in E. coli was low with either system. The deletion of a part of leader sequence from the translational initiation signal to the VSV N-gene resulted in at least 30-fold increase in production of the VSV N-protein. The VSV N-protein in E. coli was also analyzed by radioimmune blot after separation of proteins by gel electrophoresis. Degraded proteins reacted with the antibody were also observed in the cell extracts.

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