Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium
Bevan, M.W.; Mason, S.E.; Goelet, P.
EMBO Journal 4(8): 1921-1926
ISSN/ISBN: 0261-4189 PMID: 16453629 Accession: 005432316
The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a c[complementary]DNA of the 3' end of tobacco mosaic virus that encodes the coat protein of this single-stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimeric 35S coat protein RNA was detected in transformed callus.