Section 6
Chapter 5,433

Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium

Bevan, M.W.; Mason, S.E.; Goelet, P.

EMBO Journal 4(8): 1921-1926


ISSN/ISBN: 0261-4189
PMID: 16453629
Accession: 005432316

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The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a c[complementary]DNA of the 3' end of tobacco mosaic virus that encodes the coat protein of this single-stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimeric 35S coat protein RNA was detected in transformed callus.

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