EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of transferred thymidine kinase genes is controlled by methylation


, : Expression of transferred thymidine kinase genes is controlled by methylation. Proceedings of the National Academy of Sciences of the United States of America 79(20): 6299-6303

Plasmid pTKx-1, containing the herpes simplex virus gene for thymidine kinase (TK) inserted into the BamHI site of plasmid pBR322, was introduced into LtK- [thymidine kinase deficient mouse fibroblasts] cells by calcium phosphate precipitation in the absence of carrier DNA. Line 101 is a TK+ derivative of Ltk- that contains multiple copies of pTKx-1 in a multimeric structure. A derivative of 101 that retained but no longer expressed the herpes simplex TK genes (termed 101BU1) and derivatives of line 101BU1 that reexpressed the genes (termed 101H1, 101HC and 101HG) were selected. The TK genes in 101BU1 were hypermethylated relative to those in the TK+ parent and derivatives. Growth of 101BU1 in the presence of the methylation inhibitor 5-azacytidine resulted in an average 13-fold increase in the number of TK+ reexpressors. DNA from 101BU1 was inactive in secondary gene transfer, whereas DNA from 101 and from TK+ reexpressors was active. These data support a causative relationship between DNA methylation and decreased gene expression. All TK+ reexpressors examined had DNA rearrangements involving TK DNA.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432329

PMID: 6183659

DOI: 10.2307/12861

PDF Full Text: Expression of transferred thymidine kinase genes is controlled by methylation


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Furuse, M.; Yokota, H., 1985: Effect of the gut microflora on chick growth and utilisation of protein and energy at different concentrations of dietary protein. The effect of the gut flora on chick growth and protein and energy utilization at a marginal dietary energy level (calculated metabolizable energy value 11.7 MJ/kg) was estimated with diets containing protein 227 or 293 g/kg. Germ-free (GF) and co...

Trinajstic, I., 1973: Duchesnea indica (Andr.) Focke (Rosaceae), a new species in Yugoslavia adventitious flora. Acta botanica Croatica: 2 261-266

Harlow, E.; Lane, D., 2006: Immunoblotting: preparing immunoprecipitated proteins. Csh Protocols 2006(3): -

Senes, A.Melo.; Sakai, V.T.; Oliveira, T.Marchini.; Machado, M.Aparecida.A.M.; Santos, C.F.; Marzola, Cóvis., 2008: Management of a multiple dentoalveolar trauma in permanent dentition with avulsion of a canine: a 4-year follow-up. Traumatic dental injuries are relatively frequent accidents that typically involve teeth in the maxillary anterior segment. The emergency treatment and the clinical decisions must be efficiently made at the time of injury, and there is a need for...

Anonymous, 1988: Premature mortality due to alcohol-related motor vehicle traffic fatalities--United States, 1987. Mmwr. Morbidity and Mortality Weekly Report 37(49): 753-755

De-Sweemer, C., 1975: Nutrition and infection. An experimental study of their interaction in Punjabi village children. Proceedings International Congress of Nutrition: th (v 2) 168-174

Veskitkul, J.; Vichyanond, P.; Pacharn, P.; Visitsunthorn, N.; Jirapongsananuruk, O., 2016: Clinical characteristics of recurrent acute rhinosinusitis in children. Recurrent acute rhinosinusitis (RARS) is defined as multiple episodes of acute rhinosinusitis in which the symptoms and signs of infection resolve completely between episodes. Limited data are available on the characteristics and preventive therap...

Chen, H-Wen.; Chen, J.J.W.; Yu, S-Liang.; Li, H-Ni.; Yang, P-Chyr.; Su, C-Mao.; Au, H-Kien.; Chang, C-Wen.; Chien, L-Wei.; Chen, C-Sheng.; Tzeng, C-Ruey., 2005: Transcriptome analysis in blastocyst hatching by cDNA microarray. BACKGROUND: Hatching is an important process for early embryo development, differentiation and implantation. However, little is known about its regulatory mechanisms. By integrating the technologies of RNA amplification and cDNA microarrays, it ha...

Vauquelin, G., 2016: Effects of target binding kinetics on in vivo drug efficacy: koff , kon and rebinding. Optimal drug therapy often requires continuing high levels of target occupancy. Besides the traditional pharmacokinetic contribution, target binding kinetics is increasingly considered to play an important role as well. While most attention has be...

Ridpath, J.F.; Bolin, S.R., 1998: Differentiation of types 1a, 1b and 2 bovine viral diarrhoea virus (BVDV) by PCR. There are two genotypes among bovine viral diarrhoea viruses (BVDV), BVDV1 and BVDV2 Within the BVD1 genotype there are two distinct subgenotypes, BVDV1a and BVDV1b. Serology and monoclonal antibody binding are used to differentiate BVDV from clas...