EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of transformation markers and suppression of tumorigenicity in human cell hybrids


, : Expression of transformation markers and suppression of tumorigenicity in human cell hybrids. European Journal of Cancer & Clinical Oncology 18(9): 845-852

Somatic human cell hybrids produced by fusion of HeLa [human cervical carcinoma] cells and diploid fibroblasts were analyzed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20- to 100-fold higher and a cloning efficiency in semisolid medium 2- to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432342

PMID: 6961038

DOI: 10.1016/0277-5379(82)90194-8

PDF Full Text: Expression of transformation markers and suppression of tumorigenicity in human cell hybrids


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Tomi, M.; Funaki, T.; Abukawa, H.; Katayama, K.; Kondo, T.; Ohtsuki, S.; Ueda, M.; Obinata, M.; Terasaki, T.; Hosoya, K-Ichi., 2003: Expression and regulation of L-cystine transporter, system xc-, in the newly developed rat retinal Müller cell line (TR-MUL). The purpose of the present study was to elucidate the expression and regulation of the L-cystine transporter, system x(c) (-), in Müller cells. In this study, newly developed conditionally immortalized rat Müller cell lines (TR-MUL) from transge...

Margulis, A.V.; Mitchell, A.A.; Gilboa, S.M.; Werler, M.M.; Mittleman, M.A.; Glynn, R.J.; Hernandez-Diaz, S., 2013: Use of topiramate in pregnancy and risk of oral clefts. To evaluate the association between monotherapy topiramate use in pregnancy and cleft lip with or without cleft palate (CL/P) in the offspring. Data from the Slone Epidemiology Center Birth Defects Study (BDS) from 1997-29 and the National Birth D...

Anonymous, 2013: Mobius syndrome in a male with XX/XY mosaicism. We report the case of a 2-year-old male with congenital symmetric facial diplegia, and bilateral paralysis of abduction of the eyes. Findings were compatible with a diagnosis of M bius syndrome. Routine G-banded chromosome analysis revealed a mosa...

Edelstein, M.P.; Wolfei, L.A.I..; Duch, D.S., 1996: Potentiation of radiation therapy by vinorelbine in non-small cell lung cancer. Seminars in Oncology 23(2 SUPPL 5): 41-47

Nelson, K.E.; Baker, G.A., 1962: The effect of the initial and supplementary sulfur dioxide treatments on Botrytis decay and fumigant injury in storage grapes. The initial SO2 fumigation before precooling was followed by 30-minute exposures to SO2 at intervals of 1-2 weeks during cold storage. Grapes stored at R.H. 95% required 15-20% more SO2 for a given exposure than those at R.H. 85%. The amount of Bo...

McDougall, A.N., 1976: Laparoscopy in the investigation of infertility. Gynaecological units in Great Britain are using the laparoscope increasingly frequently to investigate the infertile woman; in this paper the need for this form of investigation is emphasized. In the Professorial Unit at Glasgow Royral Infirmary,...

Flood, L.M.; Kenyon, G., 2013: Surgery of otosclerosis in the Archives of The Journal of Laryngology & Otology. Journal of Laryngology and Otology 126(12): 1195-1199

Losco, P.; Troup, C., 1988: Corneal dystrophy in Fisher 344 rats. A spontaneous degenerative lesion of the cornea resembling calcific band keratopathy in man has been observed in 10-15% of the F-344 rats (aged 35-300 days) purchased from a private vendor's closed breeding colony. The lesion appears clinical...

Milner, M.S.; Liebmann, J.M.; Tello, C.; Speaker, M.G.; Ritch, R., 1994: High-resolution ultrasound biomicroscopy of the anterior segment in patients with dense corneal scars. Preoperative evaluation and surgical planning for penetrating keratoplasty in patients with dense corneal scars is often difficult because of poor visualization of the anterior segment. We performed ultrasound biomicroscopy (50-megahertz frequency...

Zhou, W.; Liu, K.; Fu, H.; Pan, K.; Zhang, L.; Wang, L.; Sun, C-Chung., 2008: Multi-modal mesoporous TiO(2)-ZrO(2) composites with high photocatalytic activity and hydrophilicity. Utilizing the amphiphilic triblock copolymer Pluronic P123 as the surfactant, and Ti(O(n)Bu)(4) and ZrOCl(2)·8H(2)O as the inorganic sources, a series of multi-modal mesoporous TiO(2)-ZrO(2) composites have been successfully synthesized through a...