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Expression of ts 3 mutant of the polyoma virus in mouse cells


Annales de Microbiologie (Paris) 130(1): 103-118
Expression of ts 3 mutant of the polyoma virus in mouse cells
A thermosensitive mutant (ts-3) of polyoma virus produces T [tumor] antigen, viral DNA and V [viral] antigen in BalbC/3T3 [mouse fibroblast] cells only at the permissive temperature (31.degree. C). At the non-permissive temperature (39.degree. C) these cells are unable to complement the viral defect. The pulse technique reveals only transitory cellular DNA induction 15-25 h after infection at 39.degree. C. At both temperatures, cytoplasmic penetration is observed and the viral DNA reaches the nuclei. The myoblast cell line PCD2 also does not complement the viral defect. In other cells tested (3T6, mouse kidney or secondary embryo cultures), the ts-3 mutant is expressed equally at 31.degree. C and 39.degree. C.


Accession: 005432353



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