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Expression of ts 3 mutant of the polyoma virus in mouse cells

, : Expression of ts 3 mutant of the polyoma virus in mouse cells. Annales de Microbiologie (Paris) 130(1): 103-118

A thermosensitive mutant (ts-3) of polyoma virus produces T [tumor] antigen, viral DNA and V [viral] antigen in BalbC/3T3 [mouse fibroblast] cells only at the permissive temperature ( C). At the non-permissive temperature ( C) these cells are unable to complement the viral defect. The pulse technique reveals only transitory cellular DNA induction 15-25 h after infection at C. At both temperatures, cytoplasmic penetration is observed and the viral DNA reaches the nuclei. The myoblast cell line PCD2 also does not complement the viral defect. In other cells tested (3T6, mouse kidney or secondary embryo cultures), the ts-3 mutant is expressed equally at C and C.

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