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Expression of tumor associated antigens by a producer and a nonproducer leukemia induced by rauscher virus


, : Expression of tumor associated antigens by a producer and a nonproducer leukemia induced by rauscher virus. Cellular Immunology 42(2): 373-383

Two Rauscher virus (R-MuLV)-induced [mouse] leukemias, RBL-5, a virus-producer, and RBL-3, a nonproducer, were compared for their expression of cell surface antigens [Ag] (RCSA), by cytotoxicity, membrane immunofluorescence, R-MuLV neutralization, and complement-fixation assays using syngeneic anti-crude membrane sera and anti-gp69/71, anti-p30 and anti-R-MuLV sera. There was a higher concentration of gp69/71 and p30 on the RBL-3 membrane than on RBL-5. This presumably is due to a cellular block at a step before assembly of virus, resulting in accumulation of these viral structural proteins (VSP). The common Ag on RBL-3 and RBL-5 is called RCSAa and may be composed of gp69/71 (RCSAa1) and an unidentified Ag (RCSAa2) which reacts more effectively with syngeneic antisera than against anti-VSP sera. An Ag abundant on the membrane of RBL-3 but not on RBL-5 is designated RCSAb, which is probably identical with p30. An unidentified Ag designated RCSAc is present on RBL-5, but not on RBL-3. Ascites cells of RBL-3 and RBL-5 exhibited less RCSA than did the cultured lines.


Accession: 005432358

PMID: 427880

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Related references

Chang, K.S.; Liu, W., 1979: Expression of tumor associated antigens by a producer and a nonproducer leukemia induced by Rauscher virus (R-MuLV). Cellular Immunology 42(2): 373-383

Schochetman, G.; Fine, D.L.; Massey, R.J., 1978: Mouse mammary tumor virus and murine leukemia virus cell surface antigens on virus producer and nonproducer mammary epithelial tumor cells. Mouse mammary tumor virus (MMTV)- and murine leukemia virus (MuLV)- specific cell surface antigens (CSA) on virus producer and nonproducer [mouse] mammary epithelial tumor cells were studied using the techniques of lactoperoxidase catalyzed iodina...

Chang, K.S., 1978: Immune reactivity of RBL-3, a nonproducer leukemia induced by Rauscher murine leukemia virus in C57BL/6 mice. A cultured line of RBL-3 leukemia, induced by Rauscher murine leukemia virus (R-MuLV) in C57BL/6 mice, was found to be a virus nonproducer. An NB tropic virus indistinguishable from R-MuLV, however, was recovered after treatment with 5-iodo-2&apos...

Galetto G.; Law L.W.; Rogers M.J., 1985: The rauscher murine leukemia virus induced leukemia rbl 5 bears two tumor associated transplantation antigens expressed on distinct molecules. Tumor cells frequently express on their surface a new antigenic determinant which renders them immunogenic in the host animal. When immunity to this antigen results in rejection of a syngeneic tumor transplant, it is referred to as a tumor-associa...

Alaba, O.; Rogers, M.J.; Law, L.W., 1979: Rauscher leukemia virus-induced tumor antigens: complete separation from gp70, p30 and H-2. The possibility that some or all of the viral proteins, gp70, p30, and the histocompatibility antigen, H-2, function as the tumor-specific transplantation antigen (TSTA) of the R-MuLV-induced leukemia, RBL-5, and also in the secondary in vitro ind...

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Ritzi E.M.; Walthall E.B., 1986: Release of mouse mammary tumor virus envelope glycoprotein from virus producer and nonproducer cells. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 324

Galetto, G.; Law, L.W.; Rogers, M.J., 1985: The Rauscher-MuLV-induced leukemia, RBL-5, bears two tumor-associated transplantation antigens expressed on distinct molecules. Tumor cells frequently express on their surface a new antigenic determinant which renders them immunogenic in the host animal. When immunity to this antigen results in rejection of a syngeneic tumor transplant, it is referred to as a tumor-associa...

Ganguly, K.; Essex, M., 1985: Feline leukemia virus-and feline sarcoma virus-related polypeptides released by virus producer and nonproducer cells. Polypeptides specific for feline leukemia virus (FeLV) have been identified in the media of cells that produce FeLV as well as in nonproducer cells transformed by feline sarcoma viruses (FeSV). Cat fibroblasts that were persistently infected with...

Rogers, M.J.; Law, L.W.; Prat, M.; Oroszlan, S.; Appella, E., 1978: Separation of the tumor rejection antigen from the major viral structural proteins associated with the membrane of an rauscher murine leukemia virus induced leukemia. TSTA was solubilized by DOC [sodium deoxycholate] from purified plasma membranes of an R-MuLV[Raucher murine leukemia virus]-induced [mouse] leukemia, RBL-5. Gel filtration chromatography resulted in the separation of high MW fractions containing...