EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS


Journal of Neuroscience 7(4): 1264-1270
Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS
In vivo, neurons of the cerebral cortex of rat embryos did not stain with antibodies to the catecholamine (CA) biosynthetic enzyme tyrosine hydroxylase (TH) even when examined using a highly sensitive technique for radioimmunocytochemistry. However, when embryonic day (E) 13 cortex was grown 1 d in culture, several thousand cells expressed immunoreactive and catalytically active TH. All TH cells simultaneously labeled with the neuronal enzyme, neuronal specific enolase, indicating that the TH was exclusively localized in neurons. Moreover, all TH neurons were postmitotic since they did not incorporate 3H-thymidine. With time in culture, the number of TH cells selectively declined from nearly 3000 cells at 2 d to several cells at 14 d. Similarly, the number of neurons competent to express TH in culture declined with advancing age of the donor embryo. Thus, by E18, very few cortical neurons had the capacity to express TH. We conclude that during a critical period of development, postmitotic cerebral cortical neurons can express catecholamine traits in vitro but not in vivo. Thus, the neutrotransmitter phenotype of certain classes of central neurons is not fixed but can be influenced by epigenetic factors found in their environment, thereby providing evidence of phenotypic plasticity in the central nervous system (CNS).


Accession: 005432385

PMID: 2883268



Related references

Plasticity of tyrosine hydroxylase gene expression in cultured neurons. Society for Neuroscience Abstracts 10(1): 358, 1984

Postnatal expression of tyrosine hydroxylase immunoreactive neurons in the cerebral cortex and striatum of the mouse treatment with disulfiram or thiram. Society for Neuroscience Abstracts 16(1): 645, 1990

Tyrosine hydroxylase-immunoreactive intrinsic neurons in the rat cerebral cortex. Experimental Brain Research 68(2): 393-405, 1987

Distribution of neurons expressing tyrosine hydroxylase in the human cerebral cortex. Journal of Anatomy 211(2): 212-222, 2007

Morphology of tyrosine hydroxylase-immunoreactive neurons in the human cerebral cortex. Experimental Brain Research 76(1): 12-20, 1989

Tyrosine hydroxylase-immunoreactive neurons in the human cerebral cortex: a novel catecholaminergic group?. Neuroscience Letters 80(3): 257-262, 1987

Lewy bodies in tyrosine hydroxylase-synthesizing neurons of the human cerebral cortex. Neuroscience Letters 106(1-2): 49-54, 1989

Tyrosine hydroxylase-immunoreactive neurons in the mouse cerebral cortex during the postnatal period. Brain Research. Developmental Brain Research 53(1): 1-5, 1990

Tyrosine hydroxylase-immunoreactive neurons are decreased in number in the cerebral cortex of Parkinson's disease. Neuropathology 19(1): 10-13, 1999

Expression of tyrosine hydroxylase and its messenger rna in cultured hypothalamic midbrain neurons. Society for Neuroscience Abstracts 15(1): 818, 1989