EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS


, : Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS. Journal of Neuroscience 7(4): 1264-1270

In vivo, neurons of the cerebral cortex of rat embryos did not stain with antibodies to the catecholamine (CA) biosynthetic enzyme tyrosine hydroxylase (TH) even when examined using a highly sensitive technique for radioimmunocytochemistry. However, when embryonic day (E) 13 cortex was grown 1 d in culture, several thousand cells expressed immunoreactive and catalytically active TH. All TH cells simultaneously labeled with the neuronal enzyme, neuronal specific enolase, indicating that the TH was exclusively localized in neurons. Moreover, all TH neurons were postmitotic since they did not incorporate 3H-thymidine. With time in culture, the number of TH cells selectively declined from nearly 3000 cells at 2 d to several cells at 14 d. Similarly, the number of neurons competent to express TH in culture declined with advancing age of the donor embryo. Thus, by E18, very few cortical neurons had the capacity to express TH. We conclude that during a critical period of development, postmitotic cerebral cortical neurons can express catecholamine traits in vitro but not in vivo. Thus, the neutrotransmitter phenotype of certain classes of central neurons is not fixed but can be influenced by epigenetic factors found in their environment, thereby providing evidence of phenotypic plasticity in the central nervous system (CNS).

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432385

PMID: 2883268

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Lange, O.; Lange, R., 1977: Darstellung der in natiirlicher Grossenordnung zu ermittelnden Oberflachenwerte rotationsellipsoidformiger Zellkerne bei Abhangigkeit vom grassen und kleinen Durchmesser der Zellkernanschnittsflache als Messgrossen, ein weiterer Beitrag zur Kernvariationsstatistik. Gegenbaurs Morphologisches Jahrbuch, 1236: 769-786

Cermeno L, G. F.; Galvan V, R.; Lobaton G, V., 1984: Life cycle and daily and seasonal population fluctuations in the sorghum midge Contarinia sorghicola (Coquillet) (Diptera: Cecidomyiidae). Observations were made in March-November 1983 of the population biology of Contarinia sorghicola in sorghum fields at 2 sites in Colombia. Most adults emerged from sorghum spikes between 06.00 and 12.00 h, with the peak emergence of males occurrin...

Rennert, H.; Zeigler-Johnson, C.; Mittal, R.Devi.; Tan, Y-cai.; Sadowl, C.M.; Edwards, J.; Finley, M.J.; Mandhani, A.; Mital, B.; Rebbeck, T.R., 2008: Analysis of the RNASEL/HPC1, and macrophage scavenger receptor 1 in Asian-Indian advanced prostate cancer. OBJECTIVES Prostate cancer (PC) varies widely by geographic location and ethnicity. American men have a high PC risk but most have localized disease. In contrast, Asian Indians have a low PC risk but most are diagnosed with metastatic disease. Ep...

Utikal, Petr; Bachleda, Petr; Kocher, Martin; Novotny, Josef; Drac, Petr; Drac, Pavel, 1999: Aneurysm of the subclavian artery. In this case report about a female patient with a true aneurysm of the left subclavian artery (SAA), the authors would like to present the symptoms, possibilities of diagnostics and management of this rarely appearing pathology. The diagnosis of a...

Kovitz, K.L.; Aleskowitch, T.D.; Sylvester, J.T.; Flavahan, N.A., 1993: Endothelium-derived contracting and relaxing factors contribute to hypoxic responses of pulmonary arteries. The response of porcine pulmonary arteries to hypoxia depended on their location in the vasculature and the degree and duration of the hypoxic challenge. In rings of pulmonary artery suspended for isometric tension recording (37 degree C, 16% O-2...

Taniguchi, T.; Kawamura, Y.; Harada, H., 1986: Relationship between deterioration and density in unearthed buried wood. Mechanical strength and density were determined for an unearthed sample of Fraxinus mandshurica (14180 BC +or- 390) and were compared with values for recent wood. Compressive and bending strengths, and density (measured by a density gradient colum...

Kruger, P.; Gunther, P. G., 1956: The sarcoplasmatic reticulum in the cross-striated muscle fibers of vertebrates and man. Early anatomists (mainly Retzius (1881, 1890)) have described in cross-striated muscle fibers of vertebrates and man a "Querfadennetz", which may be stained with goldchlorid. It was recently shown by the aid of the electron-microscope th...

Bennett-Clarke, C. A.; Mooney, R. D.; Chiaia, N. L.; Rhoades, R. W., 1991: Serotonin immunoreactive neurons are present in the superficial layers of the hamster's but not the rat's superior colliculus. Immunocytochemistry for serotonin (5-HT) was carried out in both hamsters and rats in order to determine whether or not 5-HT-positive cells existed in the superior colliculus (SC) of either species. In both hamster and rat, the superficial and dee...

Nalle, D. J.; Montgomery, C. A.; Arthur, J. L.; Polasky, S.; Schumaker, N. H., 2004: Modeling joint production of wildlife and timber. Production of marketed commodities and protection of natural systems often conflict. A focus on only one goal can result in large losses in other goals and may result in inefficient and unsustainable outcomes. In this paper, we develop a method th...

Natcheva, Rayna; Tsakiri, Evdoxia; Dihoru, Gheorghe, 2007: New bryophyte records in the Balkans: 1. New chorological data are presented for 24 species and subspecies of bryophytes from Bulgaria (reports no. 2, 3, 6, 12-24), Greece, the Rhodes island (1, 4, 5, 7), and Romania (8-11). Six taxa are liverworts (Marchantiophyta) belonging to the fam...