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Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver


, : Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver. Vestnik Akademii Meditsinskikh Nauk SSSR (6): 40-45

Gene expression in normal, neoplastic and regenerating liver was assayed by methods designed to measure the complexity of nonreiterated sequence transcripts in nuclei and polysomes of these tissues. Studies were performed comparing nuclear and total polysomal RNA of sham-operated and subtotally hepatectomized rats at 3, 6 and 20 h after surgery. The complexity of total polysomal RNA nonreiterated sequence transcripts of 3 and 6 h regenerating liver appears to be greater than that of the control tissues. At 20 h the complexities of the polysomal RNA from regenerating and control livers are not distinguishable, probably due to an increase in the complexity of the sham-operated liver polysomal RNA. The hepatoma nuclei contain quantitatively fewer sequence transcripts than do the liver nuclei from the tumor-bearing rats. In the case of the more rapidly growing E1 hepatoma, the magnitude of differences in hybridization between its nuclear RNA transcripts and those of the host liver suggests that its complexity is less than that from host liver nuclei. Hepatoma 5123 total, free and membrane-bound polysomal RNA all appeared to contain a greater variety of nonreiterated sequence transcripts than did the respective host liver polysomal RNA preparations. As a partial check of these results, sequences complimentary to those of E1-hepatoma free polysomal RNA were concentrated approximately 30-fold in a preparation of radioactively labeled nonreiterated sequence DNA. This DNA showed no significant contamination by reiterated sequences and annealed to a significantly greater degree with the tumor free polysomal RNA than with RNA from free polysomes of host liver.


Accession: 005432388

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Related references

Garrett, C.T.; Gonzalez, F.J.; Caine, M.; Kilner, J.; Wiener, D.; Genta, V.M., 1980: Expression of nonreiterated sequence transcripts in transplantable hepatomas and regenerating rat liver. Vestnik Akademii Meditsinskikh Nauk SSSR: 40-45

Garrett C.T.; Gonzalez F.J.; Kilner J., 1978: Nonreiterated sequence transcripts in 2 rat transplantable hepatomas. Federation Proceedings 37(3): 749

Roth, J.S.; Hsieh, L.L.; Peraino, C.; Weinstein, I.B., 1990: Isolation of a complementary DNA encoding the catalytic subunit of protein kinase A and studies on the expression of this sequence in rat hepatomas and regenerating liver. A complementary DNA (cDNA) clone (B4) encoding the catalytic subunit of a cAMP-dependent protein kinase (PKAc) was isolated from a lambda gt10 rat brain cDNA library, using a synthetic oligonucleotide probe whose sequence was based on the known am...

Austin G.E.; Russo R.J.; Moyer G.H., 1978: Comparison of transcripts of unique dna sequences in hepatomas induced by n 2 acetylamino fluorene and in rat liver. Federation Proceedings 37(6): 1402

Walter, V.P.; Kloppel, T.M.; Deimling, I.G.; Morré, D.J., 1980: Alterations in neutral glycosphingolipids from transplantable hepatomas and in sera of rats bearing transplantable hepatomas. Elevations in total neutral glycosphingolipids (NGL) of 5-to 30-fold were found in transplantable hepatomas when compared to control liver. Elevations of 5-to 9-fold in NGL were observed from sera of rats bearing transplantable hepatomas when comp...

Gobeev, V.N.; Davydova, S.Ia.; Khripach, L.V.; Shapot, V.S., 1976: Activity of soluble and bound hexokinase in transplantable hepatomas of different degrees of malignancy, and in normal and regenerating mouse livers. Doklady Akademii Nauk SSSR 226(5): 1210-1213

Campo, M.S.; Bishop, J.O., 1974: Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts. Journal of Molecular Biology 90(4): 649-663

Harry D.S.; Morris H.P.; Mcintyre N., 1971: The regulation of cholesterol biosynthesis in transplantable hepatomas evidence for an impairment in the uptake and storage of dietary cholesterol by transplantable hepatomas. Digestion 4(3): 150

Felder, M.R.; Church, W.K.; Shaffer, J.B., 1982: Analysis of genetically defined liver enzymes in transplantable hepatomas and developing liver of the mouse. The activities and isozyme patterns of seven enzymes involved in glycolysis, the malate-aspartate shuttle and the pentose phosphate pathway have been examined in five transplantable murine hepatomas, adult liver and fetal liver. The liver marker e...

Abe, H.; Kurata, Y., 1974: Spin concentration per living cell of normal liver, regenerating liver, and hepatomas. Gan 65(1): 75-78