EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver


, : Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver. Vestnik Akademii Meditsinskikh Nauk SSSR (6): 40-45

Gene expression in normal, neoplastic and regenerating liver was assayed by methods designed to measure the complexity of nonreiterated sequence transcripts in nuclei and polysomes of these tissues. Studies were performed comparing nuclear and total polysomal RNA of sham-operated and subtotally hepatectomized rats at 3, 6 and 20 h after surgery. The complexity of total polysomal RNA nonreiterated sequence transcripts of 3 and 6 h regenerating liver appears to be greater than that of the control tissues. At 20 h the complexities of the polysomal RNA from regenerating and control livers are not distinguishable, probably due to an increase in the complexity of the sham-operated liver polysomal RNA. The hepatoma nuclei contain quantitatively fewer sequence transcripts than do the liver nuclei from the tumor-bearing rats. In the case of the more rapidly growing E1 hepatoma, the magnitude of differences in hybridization between its nuclear RNA transcripts and those of the host liver suggests that its complexity is less than that from host liver nuclei. Hepatoma 5123 total, free and membrane-bound polysomal RNA all appeared to contain a greater variety of nonreiterated sequence transcripts than did the respective host liver polysomal RNA preparations. As a partial check of these results, sequences complimentary to those of E1-hepatoma free polysomal RNA were concentrated approximately 30-fold in a preparation of radioactively labeled nonreiterated sequence DNA. This DNA showed no significant contamination by reiterated sequences and annealed to a significantly greater degree with the tumor free polysomal RNA than with RNA from free polysomes of host liver.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432388

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Zolotarev V. N., 1968: New data on late Triassic volcanism in the central Crimean mountains. A rich upper Triassic pelecypod fauna was found in a sequence of alternating lava flows and argillites in the middle of a tuffaceous series in the southern Crimea (USSR). This sequence had earlier been placed in the Jurassic. Volcanic rocks along...

Dobriak, Ds; Rozumnyi, Ia; Luhina, Vf; Derevyts'-Kyi, Av, 1979: Organization of a raw material b. Tvarynnytstvo Ukrainy: (8) 22-23

Miettinen, Otto; Niemela, Tuomo; Ryvarden, Leif, 2007: A new polypore Irpex cremicolor described from North Europe. Irpex cremicolor, a poroid basidiomycete, is described as new based on collections from old-growth forests in northern Finland and Norway. It is characterised by a resupinate habit, lacerate pores, and a dimitic hyphal system. Most of the septa ar...

Coveney J.R.; Sparber S.B., 1982: Phencyclidine retards autoshaping at a dose which does not suppress the required response. Four groups of 5 food-deprived hooded Long-Evans rats were injected s.c. with saline (vehicle) or 2, 4 or 8 mg phencyclidine (PCP) hydrochloride/kg [a drug widely abused by humans] 15 min before being placed for the 1st time into operant chambers...

Danilova, L. K., 1958: The dynamics of cortical switching of a defensive reflex to a conditioned alimentary reflex. The elaboration of a conditioned alimentary reflex to an electro-defensive stimulus passed through 3 stages: (a) a stage of defensive responses to the current; (b) a stage of bi-effector responses; and (c) a stage of consolidation of the condition...

Hayashi, A.; Hatsukade, M., 1966: A screening technique of repellents of cockroaches. The following is based largely on the English summary. Filter paper impregnated with repellent at a prescribed concentration was inserted into paper tubes (10x3 cm.), which were placed with an empty paper tube in plastic cages into which adults of...

John Klaus W., 1981: The first phase of geologic and geotechnical reconnaissance for the crosstown tunnel of San Francisco, California. Rock Mechanics Supplementum = Felsmechanik

Fuxe, Kjell; Diaz, Rochellys; Cintra, Antonio; Bhatnagar, Maheep; Tinner, Barbro; Gustafsson, Jan Ake; Ogren, Sven Ove; Agnati, Luigi F., 1996: On the role of Glucocorticoid receptors in brain plasticity. 1. The mapping of glucocorticoid receptors (GR) in the rat central nervous system (CNS) has demonstrated their widespread presence in large numbers of nerve and glial cell populations also outside the classical stress regions. 2. The present paper...

Beugnon G., 1983: Terrestrial and celestial cues in visual orientation of the wood cricket nemobius sylvestris. Adult wood-crickets (Nemobius sylvestris), from a population living at a north-south edge between wood and field, return toward their natural habitat when they are released a few meters inside a field during the daytime. The insects are guided by...

Sacrez, R.; Fruhling, L.; Levy, J. M.; Korn, R., 1960: Leukemia with eosinophiles. A girl, 13 years of age, suffered from leuko-blastosis noted in the marrow, definite in the peripheral blood, with a very large number of adult and immature eosinophiles, often dysmorphic. The disease developed over a period of 5 months and was no...