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Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver

, : Expression of unique sequence transcripts in transplantable hepatomas and in regenerating rat liver. Vestnik Akademii Meditsinskikh Nauk SSSR (6): 40-45

Gene expression in normal, neoplastic and regenerating liver was assayed by methods designed to measure the complexity of nonreiterated sequence transcripts in nuclei and polysomes of these tissues. Studies were performed comparing nuclear and total polysomal RNA of sham-operated and subtotally hepatectomized rats at 3, 6 and 20 h after surgery. The complexity of total polysomal RNA nonreiterated sequence transcripts of 3 and 6 h regenerating liver appears to be greater than that of the control tissues. At 20 h the complexities of the polysomal RNA from regenerating and control livers are not distinguishable, probably due to an increase in the complexity of the sham-operated liver polysomal RNA. The hepatoma nuclei contain quantitatively fewer sequence transcripts than do the liver nuclei from the tumor-bearing rats. In the case of the more rapidly growing E1 hepatoma, the magnitude of differences in hybridization between its nuclear RNA transcripts and those of the host liver suggests that its complexity is less than that from host liver nuclei. Hepatoma 5123 total, free and membrane-bound polysomal RNA all appeared to contain a greater variety of nonreiterated sequence transcripts than did the respective host liver polysomal RNA preparations. As a partial check of these results, sequences complimentary to those of E1-hepatoma free polysomal RNA were concentrated approximately 30-fold in a preparation of radioactively labeled nonreiterated sequence DNA. This DNA showed no significant contamination by reiterated sequences and annealed to a significantly greater degree with the tumor free polysomal RNA than with RNA from free polysomes of host liver.

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