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Expression of v src and chicken c src in rat cell demonstrates qualitative differences between pp 60 v src and pp 60 c src

, : Expression of v src and chicken c src in rat cell demonstrates qualitative differences between pp 60 v src and pp 60 c src. Cell 37(1): 131-140

The retroviral oncogene v-src. The similarity between these genes raised the possibility that c-src might be able to elicit neoplastic growth. A chimeric plasmid was constructed that allows the expression of chicken c-src. A rat cell line containing 10 times the normal intracellular level of pp60c-src was isolated after transfecting rat-2 cells with the chimeric DNA. These cells produce the protein encoded by c-src (pp60c-src) in quantities at least 3 times greater than required to achieve transformation by the product of v-src (pp60v-src). The cells remain phenotypically normal, contain actin cables and do not grow in soft agar. Transfection of the cell line containing elevated cells of pp60c-src or Rat-2 cells with a molecular clone of v-src produces cells that exhibit properties of biologically transformed cells; round morphology, disrupted actin cables and ability to grow in soft agar.

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