EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of v src and chicken c src in rat cell demonstrates qualitative differences between pp 60 v src and pp 60 c src






Cell 37(1): 131-140

Expression of v src and chicken c src in rat cell demonstrates qualitative differences between pp 60 v src and pp 60 c src

The retroviral oncogene v-src. The similarity between these genes raised the possibility that c-src might be able to elicit neoplastic growth. A chimeric plasmid was constructed that allows the expression of chicken c-src. A rat cell line containing 10 times the normal intracellular level of pp60c-src was isolated after transfecting rat-2 cells with the chimeric DNA. These cells produce the protein encoded by c-src (pp60c-src) in quantities at least 3 times greater than required to achieve transformation by the product of v-src (pp60v-src). The cells remain phenotypically normal, contain actin cables and do not grow in soft agar. Transfection of the cell line containing elevated cells of pp60c-src or Rat-2 cells with a molecular clone of v-src produces cells that exhibit properties of biologically transformed cells; round morphology, disrupted actin cables and ability to grow in soft agar.


Accession: 005432393

PMID: 6426802



Related references

Parker, R.C.; Varmus, H.E.; Bishop, J.M., 1984: Expression of v-src and chicken c-src in rat cells demonstrates qualitative differences between pp60v-src and pp60c-src. The retroviral oncogene v-src arose by transduction of the cellular gene c-src. The similarity between these genes raised the possibility that c-src might be able to elicit neoplastic growth. We explored this by constructing a chimeric plasmid tha...

Jasoni, C.; Hendrickson, A.; Roelink, H., 1999: Analysis of chicken Wnt-13 expression demonstrates coincidence with cell division in the developing eye and is consistent with a role in induction. We used a degenerate polymerase chain reaction (PCR) strategy to search for Wnt RNA in developing ocular tissues. We isolated a Macaca monkey Wnt-13 PCR fragment, orthologous to the human and murine Wnt-13 and Xenopus Wnt-2b, and a chick Wnt13 cDN...

Velcich, A.; Corner, G.; Paul, D.; Zhuang, M.; Mariadason, J.M.; Laboisse, C.; Augenlicht, L., 2005: Quantitative rather than qualitative differences in gene expression predominate in intestinal cell maturation along distinct cell lineages. Several cell types are present in the intestinal epithelium that likely arise from a common precursor, the stem cell, and each mature cell type expresses a unique set of genes that characterizes its functional phenotype. Although the process of di...

Sawtell, N.M.; Thompson, R.L., 2004: Comparison of herpes simplex virus reactivation in ganglia in vivo and in explants demonstrates quantitative and qualitative differences. The in vivo ganglionic environment directs the latent herpes simplex virus transcriptional program. Since stress-driven perturbations in sensory neurons are thought to play a critical role in the transition from latency to reactivation, a primary...

Bommhardt, U.; Sadlack, B.; Schimpl, A., 1992: Quantitative and qualitative differences in IL-2 and IL-4 expression in primary and secondary T cell stimulation. Lymphocytes were concanavalin A (Con A) primed and the signal was withdrawn 4-48 h post-stimulation by alpha-methyl-D-mannopyranoside (alpha MM) treatment. Upon restimulation IL-2 and IL-4 RNA expression was found to be greatly enhanced. Re-expres...

Y.L.; Zhao, J.; Zhang YanLing; Liu Bin; Zhang ShouFeng; Wang FengYang; Zhao BaoQuan; H.R.ngLiang; Yin Zhen, 2001: Construction of the expressing vector of 5' flanking regulatory regions of the chicken ovalbumin gene and its transient expression in chicken primary oviduct cell and chicken fibroblasts cell cultures. The 1.1 kb region of the cloned fragment, consisting of the flanking regulatory regions was combined with the chloramphenicol acetyltransferase reporter gene (CAT gene) and an expression vector, pCAT-3-OVP was successfully constructed, pCAT-3-OVP...

Garcia-Morales, C.; Nandi, S.; Zhao, D.; Sauter, K.A.; Vervelde, L.; McBride, D.; Sang, H.M.; Clinton, M.; Hume, D.A., 2015: Cell-autonomous sex differences in gene expression in chicken bone marrow-derived macrophages. We have identified differences in gene expression in macrophages grown from the bone marrow of male and female chickens in recombinant chicken M-CSF (CSF1). Cells were profiled with or without treatment with bacterial LPS for 24 h. Approximately 6...

Rosenquist, G.L.; Gilden, R.V., 1963: Qualitative differences of chromatographic fractions of chicken antibody. FED PROC 22(2 Pt 1): 325

N├Ątt, D.; Agnvall, B.; Jensen, P., 2014: Large sex differences in chicken behavior and brain gene expression coincide with few differences in promoter DNA-methylation. While behavioral sex differences have repeatedly been reported across taxa, the underlying epigenetic mechanisms in the brain are mostly lacking. Birds have previously shown to have only limited dosage compensation, leading to high sex bias of Z-c...

Baluda, M.A.; Shoyab, M.; Evans, R.; Markham, P.D.; Ali, M., 1975: Quantitative and qualitative differences in DNA complementary to avian myeloblastosis virus between normal and leukemic chicken cells. Hybridization of avian myeloblastosis virus (AMV) RNA with DNA immobilized on filters or in liquid with a vast DNA excess was used to measure the viral specific DNA sequences in chicken cells. Newly synthesized viral DNA (v-DNA) appears within an...