EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression of variant von willebrand factor vwf complementary dna in heterologous cells requirement of the pro polypeptide in vwf multimer formation


, : Expression of variant von willebrand factor vwf complementary dna in heterologous cells requirement of the pro polypeptide in vwf multimer formation. EMBO (European Molecular Biology Organization) Journal 6(10): 2885-2890

Von Willebrand factor (vWF) is a multimeric plasma glycoprotein synthesized by vascular endothelial cells as a pre-pro-polypeptide with a highly repetitive domain structure, symbolized by the formula: (H)-D1-D2-D'-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2-(OH) a heterologous expression system for the synthesis of recombinant vWF protein was developed, consisting of a monkey kidney cell line (COS-1), transfected with full-length vWF cDNA. This system was shown to mimic the constitutive secretory pathway of vWF in endothelial cells, since dimerization and multimerization occur similarly. To determine whether the pro-polypeptide, composed of the domains D1 and D2, is involved in vWF multimerization, a vWF cDNA was constructed that lacked the coding sequence for the pro-polypeptide. The mutant vWF protein, expressed by COS-1 cells transfected with this cDNA, did not assemble beyond the dimer stage. From this observation, we conclude that (i) dimerization does not involve the pro-polypeptide of pro-vWF and (ii) the presence of the pro-polypeptide, as part of pro-vWF, is obligatory for multimerization. It is argued that the interactions, required for interchain binding, are mediated by the D domains.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432398

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Bengone Ndong, T.; Alvinerie, M., 2004: Antiparasitic macrolides: general pharmacological properties and usage instructions in the African veterinary context. Antiparasitic macrolides (or endectocides) represent the most recent class of antiparasitic drugs. This class is homogeneous because of its unique and specific, glutaminergic, mode of action, and its pharmacological properties. These drugs have a...

Mclaughlin, Joselyn; Beylottei, Frank II.; Churchwell, John; Powell, D. A., 1997: Conditioning-induced reflex facilitation in rabbits with medial prefrontal damage. Society for Neuroscience Abstracts 23(1-2): 1619

Pratt, J. H.; Longcope, C., 1978: Effect of acth on production rates and metabolic clearance rates of testosterone and estradiol. Using the constant infusion technique [7-3H]testosterone and [4-14C]estradiol were infused into 6 men and 2 women and [7-3H]androstenedione and [4-14C]estrone were infused into 2 men before and after the administration of 60 U [units] ACTH every 1...

Peradze, Rv, 1975: Concerning the formation of the gumbrin group of bentonitic clay deposits. Soobshchenieiia Akademii nauk Gruzinskoaei SSR: 78 (1) 113-115

Varshney, R. K., 1995: Genera of the diaspidoid scale insects (Coccoidea: Diaspidoidea) of South Asia. The diaspidoid scale insects of South Asia belong to 112 genera in the Halimococcidae (1 genus) and the Diaspididae (116 genera). A check-list of these genera is given. Numbers in parenthesis indicate the number of species in each genus in the reg...

Weaver S.S.; Hirshfield H.I., 1976: The delineation of 2 plankton communities from 1 sampling site fire island inlet long island new york usa. Marine Biology (Berlin) 34(3): 273-283

Yokoyama, Wayne M.; Reichlin, Amy; Margulies, David H.; Smith, Hamish R. C., 1996: MHC class I-dependent and -independent NK cell specificity. Moretta, L [Editor] Chemical Immunology; Molecular basis of NK cell recognition and function : 1-18

Zoback, Mark D.; Prescott, William H.; Krueger, Scot W., 1985: Evidence for lower crustal ductile strain localization in southern New York. Nature 317(6039): 705-707

Dear J. F., 1994: Regional concepts for gold mineralisation in the southern Connors Arch. Pages 29 1994

Zumpt F., 1970: Phumosia spangleri a new species from uganda and re description of phumosia lesnei from mozambique diptera sarcophagidae calliphorinae. Novos Taxa Entomologicos (81): 3-7