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Expression on cultured human tumor cells of placental tropho blast membrane antigens and placental alkaline phosphatase defined by mono clonal antibodies






International Journal of Cancer 30(1): 21-26

Expression on cultured human tumor cells of placental tropho blast membrane antigens and placental alkaline phosphatase defined by mono clonal antibodies

The cellular reactivity of 6 monoclonal antibodies (McAb) produced to isolated human placental syncytiotrophoblast microvillous plasma membranes was examined in a variety of normal and malignant cell types. Two McAb reacted with antigenic determinants common to most normal human cells. Two other McAb (H310 and H316) reacted predominantly with normal placentral trophoblast and with lymphocytic cells, as well as with most transformed or neoplastic cultured cell lines. Two further McAb (H315 and H317) identified fetal differentiation antigens expressed only on the membranes of normal placental trophoblast and of certain tumor cell lines. H317 was shown to be specific for the heat-stable L-phenylalanine-inhibitable placental-type alkaline phosphatase isoenzyme. These latter McAb (H315 and H317) may be useful in monitoring of some human cancers.


Accession: 005432447

PMID: 7118296



Related references

McLaughlin, P.J.; Cheng, M.H.; Slade, M.B.; Johnson, P.M., 1982: Expression on cultured human tumour cells of placental trophoblast membrane antigens and placental alkaline phosphatase defined by monoclonal antibodies. The cellular reactivity of six monoclonal antibodies (McAbs) produced to isolated human placental syncytiotrophoblast microvillous plasma membranes has been examined using a variety of normal and malignant cell types. Two McAbs reacted with antige...

Travers, P.; Bodmer, W., 1984: Preparation and characterization of mono clonal antibodies against placental alkaline phosphatase ec 3.1.3.1 and other human tropho blast associated determinants. Monoclonal antibodies were prepared by immunizing BALB/c mice with purified human term placental plasma membranes. The antibodies were selected to show predominant specificity for trophoblast and trophoblast derivatives. Four antibodies recognized...

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