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Expression on cultured human tumor cells of placental tropho blast membrane antigens and placental alkaline phosphatase defined by mono clonal antibodies


, : Expression on cultured human tumor cells of placental tropho blast membrane antigens and placental alkaline phosphatase defined by mono clonal antibodies. International Journal of Cancer 30(1): 21-26

The cellular reactivity of 6 monoclonal antibodies (McAb) produced to isolated human placental syncytiotrophoblast microvillous plasma membranes was examined in a variety of normal and malignant cell types. Two McAb reacted with antigenic determinants common to most normal human cells. Two other McAb (H310 and H316) reacted predominantly with normal placentral trophoblast and with lymphocytic cells, as well as with most transformed or neoplastic cultured cell lines. Two further McAb (H315 and H317) identified fetal differentiation antigens expressed only on the membranes of normal placental trophoblast and of certain tumor cell lines. H317 was shown to be specific for the heat-stable L-phenylalanine-inhibitable placental-type alkaline phosphatase isoenzyme. These latter McAb (H315 and H317) may be useful in monitoring of some human cancers.

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