+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression sites and developmental regulation of genes encoding 1 3 1 4 beta glucanases in germinated barley

Planta (Heidelberg) 173(4): 500-508

Expression sites and developmental regulation of genes encoding 1 3 1 4 beta glucanases in germinated barley

Expression sites of genes encoding (1 .fwdarw. 3,1 .fwdarw. 4)-.beta.-glucan 4-glycanohydrolase (EC have been mapped in germinated barley grains (Hordeum vulgare L.) by hybridization histochemistry. A 32P-labelled CDNA (copy DNA) probe was hybridized to cryosections of intact barley grains to localize complementary mRNAs. No mRNA encoding (1 .fwdarw. 3,1 .fwdarw.4)-.beta.-glucanase genes is first detected in the scutellum after 1 d and is confined to the epithelial layer. At this stage, no expression is apparent in the aleurone. After 2d, levels of (1.fwdarw. 3,1 .fwdarw. 4)-.beta.-glucanase mRNA decrease in the scutellar epithelium but increase in the aleurone. In the aleurone layer, induction of (1 .fwdarw. 3,1 .fwdarw. 4)-.beta.-glucanase gene expression, as measured by mRNA accumulation, progresses from the proximal to distal end of the grain as a front moving away from, and parallel to, the face of the scutellum.

(PDF 0-2 workdays service: $29.90)

Accession: 005432463

Related references

Expression sites and developmental regulation of genes encoding (1→3,1→4)-β-glucanases in germinated barley. Planta 173(4): 500-508, 1988

The mechanism of action of malt -glucanases VI Hydrolysis of barley -D-glucan by endo- -glucanases from germinated barley. 1964

Structure and expression of genes encoding chitinases and 1,3-beta-glucanases in potato. Plant Physiology (Rockville) 105(1 SUPPL ): 154, 1994

The mechanism of action of malt -glucanases V Separation of the -glucanases of germinated barley by dialysis and ion-exchange chromatography on phosphorylated cellulose. 1964

The mechanism of action of malt beta-glucanases IV The preparation and properties of laminarinase from germinated barley. 1963

Developmental and pathogen-induced expression of three barley genes encoding lipid transfer proteins. Plant Journal 4(6): 983-991, 1993

Regulation of genes encoding beta -D-glucan glucohydrolases in barley (Hordeum vulgare). Physiologia Plantarum 113(1): 108-120, 2001

Structure and tissue-specific regulation of genes encoding barley (1----3, 1----4)-beta-glucan endohydrolases. Molecular & General Genetics 224(3): 437-449, 1990

Regulation of expression of genes encoding phosphate transporters in barley roots. Plant nutrition molecular biology and genetics Proceedings of the Sixth International Symposium on Genetics and Molecular Biology of Plant Nutrition, Elsinore, Denmark, 17-21 August 1998: 145-150, 1999

Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.). Plant Physiology 106(4): 1705-1706, 1994