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Expression sites and developmental regulation of genes encoding 1 3 1 4 beta glucanases in germinated barley


, : Expression sites and developmental regulation of genes encoding 1 3 1 4 beta glucanases in germinated barley. Planta (Heidelberg) 173(4): 500-508

Expression sites of genes encoding (1 .fwdarw. 3,1 .fwdarw. 4)-.beta.-glucan 4-glycanohydrolase (EC 3.2.1.73) have been mapped in germinated barley grains (Hordeum vulgare L.) by hybridization histochemistry. A 32P-labelled CDNA (copy DNA) probe was hybridized to cryosections of intact barley grains to localize complementary mRNAs. No mRNA encoding (1 .fwdarw. 3,1 .fwdarw.4)-.beta.-glucanase genes is first detected in the scutellum after 1 d and is confined to the epithelial layer. At this stage, no expression is apparent in the aleurone. After 2d, levels of (1.fwdarw. 3,1 .fwdarw. 4)-.beta.-glucanase mRNA decrease in the scutellar epithelium but increase in the aleurone. In the aleurone layer, induction of (1 .fwdarw. 3,1 .fwdarw. 4)-.beta.-glucanase gene expression, as measured by mRNA accumulation, progresses from the proximal to distal end of the grain as a front moving away from, and parallel to, the face of the scutellum.

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