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Expression vectors based on the rac fusion promoter


, : Expression vectors based on the rac fusion promoter. Gene (Amsterdam) 42(1): 97-100

The -35 region of the rrnB P2 promoter and the -10 region of the lacZpo promoter-opreator were fused to form the strong and regulatable rac promoter. Vectors were constructed that allow the attachment of protein-coding sequences to the .beta.-galactosidase .alpha.-peptide (LacZ.alpha.) in any reading frame. By introducing a high-copy-number mutation, the synthesis of a LacZ.alpha.-chloramphenicol acetyltransferase fusion protein reached more than 60% of total cell protein in Escherichia coli.

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Accession: 005432469

PMID: 3087820

DOI: 10.1016/0378-1119(86)90154-X

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