EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Expression vectors based on the rac fusion promoter


, : Expression vectors based on the rac fusion promoter. Gene (Amsterdam) 42(1): 97-100

The -35 region of the rrnB P2 promoter and the -10 region of the lacZpo promoter-opreator were fused to form the strong and regulatable rac promoter. Vectors were constructed that allow the attachment of protein-coding sequences to the .beta.-galactosidase .alpha.-peptide (LacZ.alpha.) in any reading frame. By introducing a high-copy-number mutation, the synthesis of a LacZ.alpha.-chloramphenicol acetyltransferase fusion protein reached more than 60% of total cell protein in Escherichia coli.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005432469

PMID: 3087820

DOI: 10.1016/0378-1119(86)90154-X

Download PDF Full Text: Expression vectors based on the rac fusion promoter


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Jahani-Asl, A.; Germain, M.; Slack, R.S., 2010: Mitochondria: joining forces to thwart cell death. Mitochondria are highly dynamic organelles that undergo constant cycles of fusion and fission. An additional level of regulation of mitochondrial function, which is particularly important in neurons, is their active transport along microtubules. R...

Rojas, F.-Sanchez; Ojeda, C.-Bosch; Pavon, J., M.C.no, 2004: Graphite furnace atomic absorption spectrometric determination of rhodium after online ion-exchange preconcentration. A method for the determination of rhodium in different samples at trace levels is presented. The investigated metal is preconcentrated on a chelafing resin microcolumn (1,5-Bis(2-pyridyl)-3-sulphophenyl methylene thiocarbonohydarzide (PSTH) immobi...

Anonymous, 2012: Fish exposed to SSRIs exhibit abnormal behavior, Baylor study finds. Fish exhibit abnormal behavior and lower levels of anxiety when exposed to selective serotonin reuptake inhibitors (SSRI), which are common drugs used to treat depression, among other disorders. The study, by Baylor University researchers and onli...

Bargiota, A.; Walterdorf, W.; Murphy, P.; Corrall, R., 2003: The effect of chronic ischaemic hypoxia on carbohydrate metabolism in human kidney in vivo. Diabetes & Metabolism 29(Hors serie 2): 4S136-4S137, August

Prasad, K.; Gupta, J.B., 1993: Influence of hydroxyl radical on rabbit airway smooth muscle chronically exposed to H2O2 in vivo. The effects of OH on the isolated tracheal smooth muscles (TSM), from control, polyethylene glycol (PEG)-glucose oxidase (GO)-, and GO + PEG catalase-treated rabbits were investigated. GO or GO + catalase were given intravenously each week for 4...

Stein, G., 1971: Naevi and radiotherapy. Strahlentherapie 142(1): 34-36

Birchler, J.A.; Veitia, R.A., 2010: The gene balance hypothesis: implications for gene regulation, quantitative traits and evolution. The gene balance hypothesis states that the stoichiometry of members of multisubunit complexes affects the function of the whole because of the kinetics and mode of assembly. Gene regulatory mechanisms also would be governed by these principles. H...

Chuvashov B. L., 1978: Sedimentation and development of the biological communities of the Kungurian basins of the Urals; its similarity and difference with Zechstein basin of the Central Europe. Pages 10 1978

Meanos Melon, E.R.; Alonso Martinez, M.P.; Diz Gomez, J.C.; Padin Iruegas, M.E.; Muizulis Alonso, M.J.; Navarrina Martinez, J.A.; Tarrazo Antelo, A.M.; Navarrina Gamez, F., 1996: Modifications in the microvascular model of the ovary applied to the suppression of the major salivary gland. Bulletin de l'Association des Anatomistes 80(251): 48-49, Dec

Naku, A.G., 1979: Syndromologic independence of amentia. On the basis of long-term clinical observations of patients with amentive states within the framework of somatogenic, infectious, intoxicational, vascular exogenous-organic, reactive and schizophrenic psychoses the author attempts to claim the nos...