EurekaMag.com logo
+ Translate

Extended culture of mouse embryo cells without senescence: inhibition by serum


, : Extended culture of mouse embryo cells without senescence: inhibition by serum. Science 236(4798): 200-202

Mouse embryo cells cultured in vitro in serum-supplemented media undergo growth crisis, resulting in the loss of genomically normal cells prior to the appearance of established, aneuploid cells lines. Mouse embryo cells established and maintained for multiple passages in the absence of serum did not exhibit growth crisis or gross chromosomal aberration. Cells cultured under these conditions were dependent on epidermal growth factor for survival. Proliferation was reversibly inhibited by serum or platelet-free plasma, suggesting that mouse embryo cultures maintained by conventional procedures are under the influence of inhibitory factors.

(PDF same-day service: $19.90)

Accession: 005432563

PMID: 3494308

DOI: 10.1126/science.3494308

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Rawson, C.; Loo, D.; Helmrich, A.; Ernst, T.; Natsuno, T.; Merrill, G.; Barnes, D., 1991: Serum inhibition of proliferation of serum-free mouse embryo cells. Serum-free mouse embryo (SFME) cells, derived in medium supplemented with insulin, transferrin, high density lipoprotein, epidermal growth factor, and fibronectin, do not undergo crisis, maintain a predominantly diploid karyotype with no detectabl...

Chang Y., 1988: Effects of oncogenes on egf requirement and serum inhibition of serum free derived mouse embryo sfme cells. Journal Of Cell Biology Part 3: 445a

Shirahata, S.; Rawson, C.; Loo, D.; Chang, Y.J.; Barnes, D., 1990: ras and neu oncogenes reverse serum inhibition and epidermal growth factor dependence of serum-free mouse embryo cells. Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are...

Lee, J-Seon.; Kim, J-Hwan.; Seo, Y-Seok.; Yang, J-Bo.; Kim, Y-Il.; Kim, H-Jin.; Lee, K-Hwan., 2013: The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture. This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle medium cultured with Vero cell...

Wells, D.J.; Stoddard, L.S.; Getz, M.J.; Moses, H.L., 1979: alpha-Amanitin and 5-fluorouridine inhibition of serum-stimulated DNA synthesis in quiescent AKR-2B mouse embryo cells. AKR-2B mouse embryo cells undergoing the serum-stimulated transition from a quiescent to a proliferating state exhibit an increase in the rate of hnRNA synthesis which appears to be mediated through an increase in the actual number of RNA polymera...

Zeng, W.; Feng, Z., 2000: Improvement of mouse early embryo development in vitro by co-culture with human oviductal epithelial cells in serum-free medium. To examine the effect of human oviductal epithelial cells on early mouse embryonic cleavage and growth in vitro. Ninety 2-cell mouse embryos were co-cultured with human oviductal epithelial cells in DMEM/Ham's F12 + 0.3% bovine serum albumin(...

Jee, B.Chul.; Jo, J.Woo.; Lee, J.Ryeol.; Suh, C.Suk.; Kim, S.Hyun.; Moon, S.Yong., 2012: Effect of trichostatin A on fertilization and embryo development during extended culture of mouse oocyte. We performed this study to investigate the effect of histone deacetylase inhibition during extended culture of in vitro matured mouse oocytes. In vitro matured mouse (BDF1) oocytes were cultured in vitro for 6, 12, and 24 h, respectively, and then...

Yan, Z.; Liang, H.; Deng, L.; Long, H.; Chen, H.; Chai, W.; Suo, L.; Xu, C.; Kuang, Y.; Wu, L.; Lu, S.; Lyu, Q., 2016: Eight-Shaped Hatching Increases the Risk of Inner Cell Mass Splitting in Extended Mouse Embryo Culture. Increased risk of monozygotic twinning (MZT) has been shown to be associated with assisted reproduction techniques, particularly blastocyst culture. Interestingly, inner cell mass (ICM) splitting in human '8'-shaped hatching blastocysts...

Kong, Z.L.ng; Yeh, F.L.ng, 1997: Cell death of serum-free mouse embryo in culture supplied with bovine serum. Funatsu, K [Editor], Shirai, Y [Editor], Matsushita, T [Editor] Animal Cell Technology : 409-413

Omar Farouk, F.Naomi.; Stott, D.; Vlad, M., 2011: Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer. This study was conducted to examine the potential for implantation and sustainable fetal development of mouse embryos cultured from the pronuclear to blastocyst stage. Pronuclear embryos from ICR mice (Harlan Sprague-Dawley) were cultured in Sydne...