Section 6
Chapter 5,433

Extension of bacteriophage lambda host range: selection, cloning, and characterization of a constitutive lambda receptor gene

de Vries, G.E.; Raymond, C.K.; Ludwig, R.A.

Proceedings of the National Academy of Sciences of the United States of America 81(19): 6080-6084


ISSN/ISBN: 0027-8424
PMID: 6091132
DOI: 10.1073/pnas.81.19.6080
Accession: 005432797

Download citation:  

A set of plasmids has been constructed that carry a constitutive lamB gene (LamBc phenotype) from Escherichia coli and that confer functional phage lambda receptors to bacteria other than E. coli. This E. coli LamBc strain has been selected to escape both maltose-inducible and glucose-repressible control. Constitutivity results from an IS-3 insertion, carrying a mobile promoter, proximal to lamB. The LamBc DNA has been cloned into both broad and narrow host-range plasmids, and the resulting pTROY plasmids have been transferred to diverse bacteria. Both Salmonella typhimurium/pTROY and Klebsiella pneumoniae/pTROY strains efficiently adsorb phage lambda; Pseudomonas aeruginosa/pTROY strains do not. Introduction of a functional E. coli LamB protein into foreign bacterial will allow these bacteria carrying pTROY plasmids to be infected by phage lambda recombinant DNA libraries, phage lambda::Tn insertion mutagenesis vectors, and in vivo lambda-packaged cosmids.

PDF emailed within 0-6 h: $19.90