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Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal rna

, : Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal rna. Biochemical Journal 169(3): 531-542

The methylated nucleotide sequances in the rRNA molecules of the following vertebrate cultured cells were compared: human (HeLa) [cervical cancer]); hamster (BHK[baby hamster kidney]/C13); mouse [fibroblasts] (L); chickembryo fibroblast; Xenopus laevis kidney. In each species the combined 18S, 28S and 5.8S molecules possess about 110-115 methyl groups, and the methylated oligonucleotides released after complete digestion of the rRNA by T1 RN encompass several hundred nucleotides. Fingerprints of the 3 mammalian methyl-labled 18S rRNA species were qualitatively indistinguishable Fingerprints of digests of 28S rRNA of hamster and mouse L-cells were extremely similar to those of HeLa cells, differing in 1 and 3 methylated oligonucleotides, respectively. Fingerprints of methyl-labeled rRNA from chick and Xenopus strongly resembled those of mammals in most respects, but differed in several oligonucleotides in both 18S and 28S rRNA. At least some of the differences between fingerprints appear to be due to single base changes or to the presence or absence of methyl groups at particular points in the primary sequence. The methylated-nucleotide sequences are probably at least 95% homologous between the rRNA molecules of the 2 most distantly related vertebrates compared, man and X. laevis.

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