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Extensive homology between poly adenylate containing messenger rna and purified nominal poly adenylate lacking messenger rna in mouse kidney


Journal of Biological Chemistry 256(10): 5104-5108
Extensive homology between poly adenylate containing messenger rna and purified nominal poly adenylate lacking messenger rna in mouse kidney
To investigate poly(A)-lacking mRNA in mouse kidney, a fraction of renal mRNA was studied that does not bind to oligo(dT)-cellulose but can be purified by benzoylated cellulose chromatography. Nominal poly(A)-lacking mRNA and poly(A)-containing mRNA have complete nucleotide sequence homology; apparently kidney does not contain mRNA that is not represented in the polyadenylated RNA fraction. Translation products directed by nominal poly(A)-lacking mRNA and poly(A)-containing mRNA are qualitatively and quantitatively similar in 1-dimensional polyacrylamide gels. [3H]c[complementary]DNA transcribed from poly(A)-containing mRNA hybridizes with its template and with nominal poly(A)-lacking mRNA to the same extent (95%) and with the same kinetics; reaction of [3H]cDNA to nominal poly(A)-lacking mRNA with the 2 mRNA populations gives the same result. The extensive homology these 2 mRNA populations share is important to the interpretation of mRNA lifetime and to the analysis of authentic poly(A)-lacking mRNA.


Accession: 005432988



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