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External leaf features of tissue cultured cultivar silvan blackberry rubus sp

External leaf features of tissue cultured cultivar silvan blackberry rubus sp

Hortscience 21(2): 306-308

'Silvan' blackberry (Rubus sp.) has 3 types of leaf hairs: multiseriate stalked, multicelled head colleters; thick-walled unicellular hairs; and setose hairs (multiseriate trichomes that taper from a stout base). In culture, 'Silvan' blackberry leaves were unfiolate, smaller, and thinner, with less cuticle and a decreased number of trichomes compared to mature leaves of greenhouse-grown plants, which were tri-or pentafoliate. Cultured leaves had permanently open stomata, raised guard cells, and an altered stomatal and trichome distribution compared to greenhouse-grown plant leaves. Stomatal index was unaffected, but leaf size in vitro was only 1% to 2% of greenhouse control leaf area. Leaves of shoots in multiplication medium were half as large as those of plantlets in rooting medium.

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Accession: 005433492

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