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Extracellular protease extracellular hemolysin and virulence in aeromonas salmonicida



Extracellular protease extracellular hemolysin and virulence in aeromonas salmonicida



Canadian Journal of Fisheries & Aquatic Sciences 41(9): 1354-1360



Possible relationships between extracellular protease and hemolysin and virulence in A. salmonicida and the possible plasmid-encoded origin of these extracellular enzyme acivities were examined. Variants, isolated from different strains, showed simultaneous loss of both protease and hemolysin. The frequency of spontaneous loss from 1 strain (0.1-0.4%) as well as the frequency of induced loss from 4 strains (0.3-18%) treated with ethidium bromide suggested a plasmid-encoded origin for these enzymes. Plasmid analyses showed no detectable loss of plasmids correlated with the loss of the extracellular activities. There was no change in the LD100 when fish were exposed to 1 virulent strain or its protease, hemolysin-negative variants indicating that these extracellular activities are not necessary for virulence or pathogenicity in the acute form of furunculosis. Both protease, hemolysin-negative and protease, hemolysin-positive clones isolated from a 2nd virulent strain treated with ethidium bromide were avirulent (LD50 increased > 4 logs). Attenuation of A. salmonicida can occur without detected loss of the extracellular enzymes, plasmids or the A-layer.

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Accession: 005434595

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