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Extraction and identification of the major metabolite of carbonyl carbon 14 methabenzthiazuron after degradation in the soil


Extraction and identification of the major metabolite of carbonyl carbon 14 methabenzthiazuron after degradation in the soil



Journal of Agricultural and Food Chemistry 25(4): 908-912



ISSN/ISBN: 0021-8561

After soil decomposition of [carbonyl-14C]methabenzthiazuron [a herbicide], the metabolite fraction was extracted and separated from the unaltered methabenzthiazuron. Previous studies showed that due to small turnover rates, only limited concentrations of metabolites could be expected. Using HPLC [high performance liquid chromatography], it was possible to isolate, concentrate and purify the major metabolite. Its chromatographic properties were identical with 1-methyl-1-(2-benzthiazolyl)urea. The characterization was confirmed by mass spectroscopy fragmentation which yielded a parent ion at m/e 207. The manifold steps of separation and purification lead to relatively high losses. After separation from methabenzthiazuron, the metabolite fraction represented 2.4% of the applied radioactivity. After fractionation on .mu.-Porasil, only 0.4% was available for MS [mass spectrometry] analysis as major metabolite. About 0.9% was separated and not identified, and 1.1% was used for TLC and LSC [liquid scintillation counter] counting or lost during evaporation and redissolving, respectively.

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Accession: 005435182

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Related references

Extraction and identification of the major metabolite of [carbonyl-14C]methabenzthiazuron after degradation in the soil. Journal of Agricultural and Food Chemistry 25(4): 908-912, 1977

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