EurekaMag
+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Factors contributing to the modulation of norepinephrine uptake by synaptosomes from mouse brain cortex


Factors contributing to the modulation of norepinephrine uptake by synaptosomes from mouse brain cortex



Brain Research 121(1): 113-120



ISSN/ISBN: 0006-8993

PMID: 832147

DOI: 10.1016/0006-8993(77)90441-3

The mode of inheritance of the synaptosomal mechanism for uptake of norepinephrine (NE) was studied in 2 inbred stains of mice, BALB/cBy and C57BL/6By, along with the reciprocal F1 hybrids and 7 recombinant inbred stains, CXBD, CXBE, CXBG, CXBH, CXBI, CXBJ and CXBK. All these strains were also tested in the open field as a measure of response to mild stress, since stress had been shown to affect the kinetic constants of synaptosomal uptake. The 2 parental strains showed a significant difference in Km for NE uptake similar to that previously reported between BALB/cJ and C57BL/10J, and no significant difference in Vmax. The F1 hybrids resembled C57BL/6By, and the recombinant inbred strains showed no significant differences from either parent with only minor exceptions. This makes further genetic analysis impossible with the data available at this time. A high positive correlation existed between Km and Vmax (r = 0.89). The affinity for NE uptake and the number of uptake sites available seemed to be modulated in a coordinated fashion. When the data on Km for all strains tested are pooled, a bimodal distribution was apparent. There were 2 populations with means of 2.25 and 4.03 .times. 10-7 M, respectively. Analysis of open field ambulation enabled the strains to be divided into a high (C57BL/6By, BXCF1, CXBF1, CXBD, CXBE and CXBK) and a low group (BALB/cBy, CXBG, CXBH, CXBI and CXBJ). There was a significant negative interstrain correlation (r = 0.87) between open field ambulation and Km for NE uptake. If open field ambulation was taken as an index of reactivity to stress (high ambulation-low reactivity and vice versa), then the data could be regrouped on NE uptake into 2 categories: 78% of the mice from the highly reactive strains presented high Km for NE uptake, while only 35% of the non-reactive mice showed high Km. The bimodal distribution was apparent in both cases and the means of both high Km groups were identical; the same is true of the means for both low Km groups of strains of mice. It appears that Km for NE uptake does not vary along a continuum but it presents 2 discrete values. This would be suggestive of the existence of 2 distinct conformational states for the presumably proteinic uptake site. Stress presumably causes a switch from the high affinity conformation to the low affinity conformation. Thus a higher percentage of individuals from strains highly reactive to stress shows low affinity for NE uptake.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 005441149

Download citation: RISBibTeXText

Related references

Effects of narcotics and other psychotropic drugs on the uptake of norepinephrine by mouse brain synaptosomes. Federation Proceedings 32(3 Part 1): 815, 1973

Effect of lithium on content and uptake of norepinephrine and 5-hydroxytryptamine in mouse brain synaptosomes and mitochondria. Life Sciences. Pt. 1: Physiology and Pharmacology 9(21): 1213-1220, 1970

Purinergic modulation of norepinephrine release and uptake in rat brain cortex: contribution of glial cells. Journal of Neurophysiology 110(11): 2580-2591, 2013

A comparison of the inhibitory effects of new non-tricyclic amine uptake inhibitors on the uptake of norepinephrine and 5-hydroxytryptamine into synaptosomes of the rat brain. Neuropharmacology 19(4): 349-354, 1980

The effect of phospholipases on the active uptake of norepinephrine by synaptosomes isolated from the cerebral cortex of guinea pig. Journal of Neurochemistry 22(4): 551-556, 1974

Effects of ions on uptake of norepinephrine by synaptosomes abstract rat brain tritiated norepinephrine metab reserpine metab potassium sodium lithium. Pharmacologist 9(2): 184, 1967

Norepinephrine uptake by cerebral synaptosomes of mouse: strain differences. Brain Research 92(1): 157-164, 1975

Effects of cadmium on the uptake of dopamine and norepinephrine in rat brain synaptosomes. Bulletin of Environmental Contamination and Toxicology 37(3): 421-426, 1986

Influence of mercury on uptake of [3H]dopamine and [3H]norepinephrine by rat brain synaptosomes. Toxicology Letters 27(1-3): 7-14, 1985

Effects of pH changes and charge characteristics in the uptake of norepinephrine by synaptosomes of rat brain. Biochimica et Biophysica Acta 540(2): 337-345, 1978

Competition by monophenolic estrogens and catecholestrogens for high-affinity uptake of [3H](-)-norepinephrine into synaptosomes from rat cerebral cortex and hypothalamus. Brain Research 277(1): 163-168, 1983

6-Nitro-norepinephrine increases norepinephrine release and inhibits norepinephrine uptake in spinal cord synaptosomes. FASEB Journal 13(5 Part 2): A806, 1999

The effect of mepiprazole on the uptake of norepinephrine, dopamine and serotonin into rat brain synaptosomes. Naunyn-Schmiedeberg'sArchivesofPharmacology287Suppl:R4, 1975

6-NO(2)-norepinephrine increases norepinephrine release and inhibits norepinephrine uptake in rat spinal synaptosomes. Journal of Pharmacology and Experimental Therapeutics 292(3): 895-899, 2000

Promotion of norepinephrine release and inhibition of calcium uptake by pyrethroids in rat brain synaptosomes. Pesticide Biochemistry and Physiology 29(3): 187-196, 1987