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Feulgen staining with picric acid and m phosphoric acid schiff reagents and their shelf life



Feulgen staining with picric acid and m phosphoric acid schiff reagents and their shelf life



Cellular & Molecular Biology (Oxford) 27(2-3): 175-180



Two modified Schiff reagents (SR) prepared with picric acid or m-phosphoric acid in place of the usual N HCl were studied. They contain the usual potassium metabisulfite. After filtration, following shaking with activated charcoal, the picric acid SR was of pale pink color, whereas that prepared with m-phosphoric acid was of a straw-colored dye-reagent. Both the dye-reagents following Feulgen staining revealed very well-stained nuclei and the shelf-life of both was at least 3 mo., much more than that of the conventional SR, prepared with N HCl and potassium metabisulfite. The absorption spectra of the pale pink-colored picric acid SR as well as those of the straw-colored m-phosphoric acid SR were also presented. A possible reason for the longer shelf-life of these newly developed modified SR was suggested, and the mechanism of action of these dye-reagents was discussed.

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Accession: 005458140

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